气候变化领域集成服务门户(CCPortal): Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures

CCPortal

DOI	10.1073/pnas.2100165118
	Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures
	Uno K.; Aktalay A.; Bossi M.L.; Irie M.; Belov V.N.; Hell S.W.
发表日期	2021
ISSN	00278424
卷号	118 期号:14
英文摘要	The use of photoswitchable fluorescent diarylethenes (fDAEs) as protein labels in fluorescence microscopy and nanoscopy has been limited by labeling inhomogeneity and the need for ultraviolet light for fluorescence activation (on-switching). To overcome these drawbacks, we prepared "turn-on mode" fDAEs featuring thienyl substituents, multiple polar residues, and a reactive maleimide group in the core structure. Conjugates with antibodies and nanobodies displayed complete on-switching and excitation with violet (405 nm) and yellow-green (<565 nm) light, respectively. Besides, they afforded high signal-to-noise ratios and low unspecific labeling in fluorescence imaging. Irradiation with visible light at 532 nm or 561 nm led to transient on-off switching ("blinking") of the fDAEs of double-labeled nanobodies so that nanoscale superresolution images were readily attained through switching and localization of individual fluorophores. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bioconjugation; Chromophores; Diarylethenes; Fluorescence; Urbachtail
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180016
作者单位	Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, 37077, Germany; Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Heidelberg, 69120, Germany; Research Center for Smart Molecules, Rikkyo University, Tokyo, 171-8501, Japan
推荐引用方式 GB/T 7714	Uno K.,Aktalay A.,Bossi M.L.,et al. Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures[J],2021,118(14).
APA	Uno K.,Aktalay A.,Bossi M.L.,Irie M.,Belov V.N.,&Hell S.W..(2021).Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures.Proceedings of the National Academy of Sciences of the United States of America,118(14).
MLA	Uno K.,et al."Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures".Proceedings of the National Academy of Sciences of the United States of America 118.14(2021).