气候变化领域集成服务门户(CCPortal): Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome

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DOI	10.1073/pnas.2024846118
	Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome
	Das M.; Zattas D.; Zinder J.C.; Wasmuth E.V.; Henri J.; Lima C.D.
发表日期	2021
ISSN	00278424
卷号	118 期号:14
英文摘要	Quality control requires discrimination between functional and aberrant species to selectively target aberrant substrates for destruction. Nuclear RNA quality control in Saccharomyces cerevisiae includes the TRAMP complex that marks RNA for decay via polyadenylation followed by helicase-dependent 3' to 5' degradation by the RNA exosome. Using reconstitution biochemistry, we show that polyadenylation and helicase activities of TRAMP cooperate with processive and distributive exoribonuclease activities of the nuclear RNA exosome to protect stable RNA from degradation while selectively targeting and degrading less stable RNA. Substrate discrimination is lost when the distributive exoribonuclease activity of Rrp6 is inactivated, leading to degradation of stable and unstable RNA species. These data support a proofreading mechanism in which deadenylation by Rrp6 competes with Mtr4- dependent degradation to protect stable RNA while selectively targeting and degrading unstable RNA. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Exosome; Quality control; RNA; RNA decay; TRAMP
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180014
作者单位	Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, United States; Tri-Institutional Training Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, United States; HHMI, Memorial Sloan Kettering Cancer Center, New York, NY 10065, United States
推荐引用方式 GB/T 7714	Das M.,Zattas D.,Zinder J.C.,et al. Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome[J],2021,118(14).
APA	Das M.,Zattas D.,Zinder J.C.,Wasmuth E.V.,Henri J.,&Lima C.D..(2021).Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome.Proceedings of the National Academy of Sciences of the United States of America,118(14).
MLA	Das M.,et al."Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome".Proceedings of the National Academy of Sciences of the United States of America 118.14(2021).