气候变化领域集成服务门户(CCPortal): A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish

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DOI	10.1073/pnas.2015748118
	A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish
	Xia J.; Kang Z.; Xue Y.; Ding Y.; Gao S.; Zhang Y.; Lv P.; Wang X.; Ma D.; Wang L.; Han J.-D.J.; Liu F.
发表日期	2021
ISSN	00278424
卷号	118 期号:14
英文摘要	During vertebrate embryogenesis, fetal hematopoietic stem and progenitor cells (HSPCs) exhibit expansion and differentiation properties in a supportive hematopoietic niche. To profile the developmental landscape of fetal HSPCs and their local niche, here, using single-cell RNA-sequencing, we deciphered a dynamic atlas covering 28,777 cells and 9 major cell types (23 clusters) of zebrafish caudal hematopoietic tissue (CHT). We characterized four heterogeneous HSPCs with distinct lineage priming and metabolic gene signatures. Furthermore, we investigated the regulatory mechanism of CHT niche components for HSPC development, with a focus on the transcription factors and ligand-receptor networks involved in HSPC expansion. Importantly, we identified an endothelial cell-specific G protein-coupled receptor 182, followed by in vivo and in vitro functional validation of its evolutionally conserved role in supporting HSPC expansion in zebrafish and mice. Finally, comparison between zebrafish CHT and human fetal liver highlighted the conservation and divergence across evolution. These findings enhance our understanding of the regulatory mechanism underlying hematopoietic niche for HSPC expansion in vivo and provide insights into improving protocols for HSPC expansion in vitro. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Caudal hematopoietic tissue; Gpr182; HSPC expansion; Single-cell RNA-seq; Zebrafish
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179998
作者单位	State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Center for Quantitative Biology, Peking University, Beijing, 100871, China; State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin, 300020, China
推荐引用方式 GB/T 7714	Xia J.,Kang Z.,Xue Y.,et al. A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish[J],2021,118(14).
APA	Xia J..,Kang Z..,Xue Y..,Ding Y..,Gao S..,...&Liu F..(2021).A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish.Proceedings of the National Academy of Sciences of the United States of America,118(14).
MLA	Xia J.,et al."A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish".Proceedings of the National Academy of Sciences of the United States of America 118.14(2021).