气候变化领域集成服务门户(CCPortal): The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila through multiple modalities

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DOI	10.1073/pnas.2010210118
	The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila through multiple modalities
	Syed S.; Wilky H.; Raimundo J.; Lim B.; Amodeo A.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:14
英文摘要	Early embryos must rapidly generate large numbers of cells to form an organism. Many species accomplish this through a series of rapid, reductive, and transcriptionally silent cleavage divisions. Previous work has demonstrated that the number of divisions before both cell cycle elongation and zygotic genome activation (ZGA) is regulated by the ratio of nuclear content to cytoplasm (N/C). To understand how the N/C ratio affects the timing of ZGA, we directly assayed the behavior of several previously identified N/C ratio- dependent genes using the MS2-MCP reporter system in living Drosophila embryos with altered ploidy and cell cycle durations. For every gene that we examined, we found that nascent RNA output per cycle is delayed in haploid embryos. Moreover, we found that the N/C ratio influences transcription through three overlapping modes of action. For some genes (knirps, fushi tarazu, and snail), the effect of ploidy can be primarily attributed to changes in cell cycle duration. However, additional N/C ratio-mediated mechanisms contribute significantly to transcription delays for other genes. For giant and bottleneck, the kinetics of transcription activation are significantly disrupted in haploids, while for frühstart and Krüppel, the N/C ratio controls the probability of transcription initiation. Our data demonstrate that the regulatory elements of N/C ratio-dependent genes respond directly to the N/C ratio through multiple modes of regulation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell cycle; Mid-blastula transition; N/C ratio; Transcription; ZGA
语种	英语
scopus关键词	fushi tarazu protein; kruppel like factor; messenger RNA; transcription factor Snail; Article; bottleneck gene; cell cycle regulation; controlled study; cytoplasm; Drosophila; embryo; Enterobacteria phage MS2; fruhstart gene; fushi tarazu gene; gene activation; gene control; gene function; gene identification; genetic transcription; giant gene; haploidy; insect genetics; knirps gene; Kruppel gene; nonhuman; overlapping gene; ploidy; priority journal; snail gene; transcription initiation; zygote
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179986
作者单位	Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104, United States; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, United States; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, United States
推荐引用方式 GB/T 7714	Syed S.,Wilky H.,Raimundo J.,et al. The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila through multiple modalities[J],2021,118(14).
APA	Syed S.,Wilky H.,Raimundo J.,Lim B.,&Amodeo A.A..(2021).The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila through multiple modalities.Proceedings of the National Academy of Sciences of the United States of America,118(14).
MLA	Syed S.,et al."The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila through multiple modalities".Proceedings of the National Academy of Sciences of the United States of America 118.14(2021).