气候变化领域集成服务门户(CCPortal): Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor

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DOI	10.1073/pnas.2023807118
	Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor
	Xia J.; Yang H.; Mu M.; Micovic N.; Poskanzer K.E.; Monaghan J.R.; Clark H.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:14
英文摘要	The ability to monitor the release of neurotransmitters during synaptic transmission would significantly impact the diagnosis and treatment of neurological diseases. Here, we present a DNA-based enzymatic nanosensor for quantitative detection of acetylcholine (ACh) in the peripheral nervous system of living mice. ACh nanosensors consist of DNA as a scaffold, acetylcholinesterase as a recognition component, pH-sensitive fluorophores as signal generators, and α-bungarotoxin as a targeting moiety. We demonstrate the utility of the nanosensors in the submandibular ganglia of livingmice to sensitively detect ACh ranging from 0.228 to 358 μM. In addition, the sensor response upon electrical stimulation of the efferent nerve is dose dependent, reversible, and we observe a reduction of ∼76% in sensor signal upon pharmacological inhibition of ACh release. Equipped with an advanced imaging processing tool, we further spatially resolve ACh signal propagation on the tissue level. Our platform enables sensitive measurement and mapping of ACh transmission in the peripheral nervous system. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Acetylcholine; Acetylcholinesterase; DNA; Nanosensor; Submandibular ganglion
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179964
作者单位	Department of Bioengineering, College of Engineering, Northeastern University, Boston, MA 02115, United States; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, United States; Kavli Insititute for Fundamental Neuroscience, San Francisco, CA 94143, United States; Department of Biology, College of Science, Northeastern University, Boston, MA 02115, United States; Department of Chemistry and Chemical Biology, College of Science, Northeastern University, Boston, MA 02115, United States
推荐引用方式 GB/T 7714	Xia J.,Yang H.,Mu M.,et al. Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor[J],2021,118(14).
APA	Xia J..,Yang H..,Mu M..,Micovic N..,Poskanzer K.E..,...&Clark H.A..(2021).Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor.Proceedings of the National Academy of Sciences of the United States of America,118(14).
MLA	Xia J.,et al."Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor".Proceedings of the National Academy of Sciences of the United States of America 118.14(2021).