气候变化领域集成服务门户(CCPortal): Virus-induced cell gigantism and asymmetric cell division in archaea

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DOI	10.1073/pnas.2022578118
	Virus-induced cell gigantism and asymmetric cell division in archaea
	Liu J.; Cvirkaite-Krupovic V.; Baquero D.P.; Yang Y.; Zhang Q.; Shen Y.; Krupovic M.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Archaeal viruses represent one of the most mysterious parts of the global virosphere, with many virus groups sharing no evolutionary relationship to viruses of bacteria or eukaryotes. How these viruses interact with their hosts remains largely unexplored. Here we show that nonlytic lemon-shaped virus STSV2 interferes with the cell cycle control of its host, hyperthermophilic and acidophilic archaeon Sulfolobus islandicus, arresting the cell cycle in the S phase. STSV2 infection leads to transcriptional repression of the cell division machinery, which is homologous to the eukaryotic endosomal sorting complexes required for transport (ESCRT) system. The infected cells grow up to 20-fold larger in size, have 8,000-fold larger volume compared to noninfected cells, and accumulate massive amounts of viral and cellular DNA. Whereas noninfected Sulfolobus cells divide symmetrically by binary fission, the STSV2-infected cells undergo asymmetric division, whereby giant cells release normal-sized cells by budding, resembling the division of budding yeast. Reinfection of the normal-sized cells produces a new generation of giant cells. If the CRISPR-Cas system is present, the giant cells acquire virus-derived spacers and terminate the virus spread, whereas in its absence, the cycle continues, suggesting that CRISPR-Cas is the primary defense system in Sulfolobus against STSV2. Collectively, our results show how an archaeal virus manipulates the cell cycle, transforming the cell into a giant virion-producing factory. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Archaea; Archaeal viruses; Asymmetric cell division; ESCRT system; Saccharolobus
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179943
作者单位	Archaeal Virology Unit, Institut Pasteur, Paris, 75015, France; CRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, 266237, China; Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China
推荐引用方式 GB/T 7714	Liu J.,Cvirkaite-Krupovic V.,Baquero D.P.,et al. Virus-induced cell gigantism and asymmetric cell division in archaea[J],2021,118(15).
APA	Liu J..,Cvirkaite-Krupovic V..,Baquero D.P..,Yang Y..,Zhang Q..,...&Krupovic M..(2021).Virus-induced cell gigantism and asymmetric cell division in archaea.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Liu J.,et al."Virus-induced cell gigantism and asymmetric cell division in archaea".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).