气候变化领域集成服务门户(CCPortal): Establishment of heterochromatin in domain-size-dependent bursts

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DOI	10.1073/pnas.2022887118
	Establishment of heterochromatin in domain-size-dependent bursts
	Nickels J.F.; Edwards A.K.; Charlton S.J.; Mortensen A.M.; Hougaard S.C.L.; Trusina A.; Sneppen K.; Thon G.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Methylation of histone H3K9 is a hallmark of epigenetic silencing in eukaryotes. Nucleosome modifications often rely on positive feedback where enzymes are recruited by modified nucleosomes. A combination of local and global feedbacks has been proposed to account for some dynamic properties of heterochromatin, but the range at which the global feedbacks operate and the exact mode of heterochromatin propagation are not known. We investigated these questions in fission yeast. Guided by mathematical modeling, we incrementally increased the size of the mating-type region and profiled heterochromatin establishment over time. We observed exponential decays in the proportion of cells with active reporters, with rates that decreased with domain size. Establishment periods varied from a few generations in wild type to >200 generations in the longest region examined, and highly correlated silencing of two reporters located outside the nucleation center was observed. On a chromatin level, this indicates that individual regions are silenced in sudden bursts. Mathematical modeling accounts for these bursts if heterochromatic nucleosomes facilitate a deacetylation or methylation reaction at long range, in a distance-independent manner. A likely effector of three-dimensional interactions is the evolutionarily conserved Swi6HP1 H3K9me reader, indicating the bursting behavior might be a general mode of heterochromatin propagation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Fission yeast; Gene silencing; Heterochromatin; Modeling
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179935
作者单位	Niels Bohr Institute, University of Copenhagen, Copenhagen, 2100, Denmark; Department of Biology, University of Copenhagen, Copenhagen, 2200, Denmark
推荐引用方式 GB/T 7714	Nickels J.F.,Edwards A.K.,Charlton S.J.,et al. Establishment of heterochromatin in domain-size-dependent bursts[J],2021,118(15).
APA	Nickels J.F..,Edwards A.K..,Charlton S.J..,Mortensen A.M..,Hougaard S.C.L..,...&Thon G..(2021).Establishment of heterochromatin in domain-size-dependent bursts.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Nickels J.F.,et al."Establishment of heterochromatin in domain-size-dependent bursts".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).