气候变化领域集成服务门户(CCPortal): Establishment of bovine expanded potential stem cells

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DOI	10.1073/pnas.2018505118
	Establishment of bovine expanded potential stem cells
	Zhao L.; Gao X.; Zheng Y.; Wang Z.; Zhao G.; Ren J.; Zhang J.; Wu J.; Wu B.; Chen Y.; Sun W.; Li Y.; Su J.; Ding Y.; Gao Y.; Liu M.; Bai X.; Sun L.; Cao G.; Tang F.; Bao S.; Liu P.; Li X.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight-cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture. © This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
英文关键词	Bovine; Expanded potential stem cell; Nuclear transfer
语种	英语
scopus关键词	agriculture; animal cell; animal experiment; article; biotechnology; bovine; cell culture; cell lineage; chimera; embryo; embryonic stem cell; female; fetus; gene editing; gene expression; germ layer; male; nonhuman; pig; preimplantation embryo; somatic cell nuclear transfer; wild type
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179915
作者单位	The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China; Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, 010070, China; Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, 011517, China; Academy of Orthopedics, Guangdong Province, Department of Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510630, China; Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China; School of Biomedical Science, Stem Cell and Regenerative Consortium, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 999077, Hong Kong; Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Peking University, Beijing, 100871, China; College of Veterinary Medicine, Key Laboratory of Basic Ve...
推荐引用方式 GB/T 7714	Zhao L.,Gao X.,Zheng Y.,et al. Establishment of bovine expanded potential stem cells[J],2021,118(15).
APA	Zhao L..,Gao X..,Zheng Y..,Wang Z..,Zhao G..,...&Li X..(2021).Establishment of bovine expanded potential stem cells.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Zhao L.,et al."Establishment of bovine expanded potential stem cells".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).