气候变化领域集成服务门户(CCPortal): Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis

CCPortal

DOI	10.1073/pnas.2021210118
	Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis
	Adar-Levor S.; Nachmias D.; Gal-Oz S.T.; Jahn Y.M.; Peyrieras N.; Zaritsky A.; Birnbaum R.Y.; Elia N.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Animal cytokinesis ends with the formation of a thin intercellular membrane bridge that connects the two newly formed sibling cells, which is ultimately resolved by abscission. While mitosis is completed within 15 min, the intercellular bridge can persist for hours, maintaining a physical connection between sibling cells and allowing exchange of cytosolic components. Although cell-cell communication is fundamental for development, the role of intercellular bridges during embryogenesis has not been fully elucidated. In this work, we characterized the spatiotemporal characteristics of the intercellular bridge during early zebrafish development. We found that abscission is delayed during the rapid division cycles that occur in the early embryo, giving rise to the formation of interconnected cell clusters. Abscission was accelerated when the embryo entered the midblastula transition (MBT) phase. Components of the ESCRT machinery, which drives abscission, were enriched at intercellular bridges post-MBT and, interfering with ESCRT function, extended abscission beyond MBT. Hallmark features of MBT, including transcription onset and cell shape modulations, were more similar in interconnected sibling cells compared to other neighboring cells. Collectively, our findings suggest that delayed abscission in the early embryo allows clusters of cells to coordinate their behavior during embryonic development. © This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Abscission; Cell division; Development; MBT; Microtubules
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179902
作者单位	Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel; National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel; BioEmergences Laboratory, USR 3695, CNRS, Université Paris-Saclay, Gif-sur-Yvette, 91198, France; Department of Software and Information Systems Engineering, Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel; Center of Evolutionary Genomics and Medicine, The Ben-Gurion University of the Negev84105, Israel
推荐引用方式 GB/T 7714	Adar-Levor S.,Nachmias D.,Gal-Oz S.T.,et al. Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis[J],2021,118(15).
APA	Adar-Levor S..,Nachmias D..,Gal-Oz S.T..,Jahn Y.M..,Peyrieras N..,...&Elia N..(2021).Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Adar-Levor S.,et al."Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).