气候变化领域集成服务门户(CCPortal): Conditional destabilization of the TPLATE complex impairs endocytic internalization

CCPortal

DOI	10.1073/pnas.2023456118
	Conditional destabilization of the TPLATE complex impairs endocytic internalization
	Wang J.; Yperman K.; Grones P.; Jiang Q.; Dragwidge J.; Mylle E.; Mor E.; Nolf J.; Eeckhout D.; de Jaeger G.; de Rybel B.; Pleskot R.; van Damme D.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	In plants, endocytosis is essential for many developmental and physiological processes, including regulation of growth and development, hormone perception, nutrient uptake, and defense against pathogens. Our toolbox to modulate this process is, however, rather limited. Here, we report a conditional tool to impair endocytosis. We generated a partially functional TPLATE allele by substituting the most conserved domain of the TPLATE subunit of the endocytic TPLATE complex (TPC). This substitution destabilizes TPC and dampens the efficiency of endocytosis. Short-term heat treatment increases TPC destabilization and reversibly delocalizes TPLATE from the plasma membrane to aggregates in the cytoplasm. This blocks FM uptake and causes accumulation of various known endocytic cargoes at the plasma membrane. Short-term heat treatment therefore transforms the partially functional TPLATE allele into an effective conditional tool to impair endocytosis. Next to their role in endocytosis, several TPC subunits are also implicated in actin-regulated autophagosomal degradation. Inactivating TPC via the WDX mutation, however, does not impair autophagy, thus enabling specific and reversible modulation of endocytosis in planta. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Arabidopsis; Autophagy; Clathrin-mediated endocytosis; Temperature-sensitive mutant; TPLATE complex
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179896
作者单位	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, 9052, Belgium; VIB Center for Plant Systems Biology, Ghent, 9052, Belgium; Institute of Experimental Botany, Czech Academy of Sciences, Prague 6, 165 02, Czech Republic
推荐引用方式 GB/T 7714	Wang J.,Yperman K.,Grones P.,et al. Conditional destabilization of the TPLATE complex impairs endocytic internalization[J],2021,118(15).
APA	Wang J..,Yperman K..,Grones P..,Jiang Q..,Dragwidge J..,...&van Damme D..(2021).Conditional destabilization of the TPLATE complex impairs endocytic internalization.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Wang J.,et al."Conditional destabilization of the TPLATE complex impairs endocytic internalization".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).