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DOI	10.1073/pnas.2022308118
	Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded rieske FeS cluster
	Zhou X.; Gao Y.; Wang W.; Yang X.; Yang X.; Liu F.; Tang Y.; Lam S.M.; Shui G.; Yu L.; Tian C.; Guddat L.W.; Wang Q.; Rao Z.; Gong H.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Complex II, also known as succinate dehydrogenase (SQR) or fumarate reductase (QFR), is an enzyme involved in both the Krebs cycle and oxidative phosphorylation. Mycobacterial Sdh1 has recently been identified as a new class of respiratory complex II (type F) but with an unknown electron transfer mechanism. Here, using cryoelectron microscopy, we have determined the structure of Mycobacterium smegmatis Sdh1 in the presence and absence of the substrate, ubiquinone-1, at 2.53-Å and 2.88-Å resolution, respectively. Sdh1 comprises three subunits, two that are water soluble, SdhA and SdhB, and one that is membrane spanning, SdhC. Within these subunits we identified a quinone-binding site and a rarely observed Rieske-type [2Fe-2S] cluster, the latter being embedded in the transmembrane region. A mutant, where two His ligands of the Rieske-type [2Fe-2S] were changed to alanine, abolished the quinone reduction activity of the Sdh1. Our structures allow the proposal of an electron transfer pathway that connects the substrate-binding and quinone-binding sites. Given the unique features of Sdh1 and its essential role in Mycobacteria, these structures will facilitate antituberculosis drug discovery efforts that specifically target this complex. © This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Cryoelectron microscopy; Electron transport chain; Mycobacterium smegmatis; Succinate dehydrogenase
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179889
作者单位	Shanghai Institute for Advanced Immunochemical Studies, School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin, 300353, China; CAS, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), Shanghai, 200031, China; University of CAS, Beijing, 100101, China; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, CAS, Beijing, 100101, China; High Magnetic Field Laboratory, CAS, Hefei, 230031, China; Hefei National Laboratory of Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia; National Laboratory of Biomacromolecules...
推荐引用方式 GB/T 7714	Zhou X.,Gao Y.,Wang W.,et al. Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded rieske FeS cluster[J],2021,118(15).
APA	Zhou X..,Gao Y..,Wang W..,Yang X..,Yang X..,...&Gong H..(2021).Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded rieske FeS cluster.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Zhou X.,et al."Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded rieske FeS cluster".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).