气候变化领域集成服务门户(CCPortal): The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3′-UTRs of yeast mitochondrial mRNAs

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DOI	10.1073/pnas.2009329118
	The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3′-UTRs of yeast mitochondrial mRNAs
	Hillen H.S.; Markov D.A.; Wojtas I.D.; Hofmann K.B.; Lidschreiber M.; Cowan A.T.; Jones J.L.; Temiakov D.; Cramer P.; Anikin M.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Stabilization of messenger RNA is an important step in posttranscriptional gene regulation. In the nucleus and cytoplasm of eukaryotic cells it is generally achieved by 5′ capping and 3′ polyadenylation, whereas additional mechanisms exist in bacteria and organelles. The mitochondrial mRNAs in the yeast Saccharomyces cerevisiae comprise a dodecamer sequence element that confers RNA stability and 3′-end processing via an unknown mechanism. Here, we isolated the protein that binds the dodecamer and identified it as Rmd9, a factor that is known to stabilize yeast mitochondrial RNA. We show that Rmd9 associates with mRNA around dodecamer elements in vivo and that recombinant Rmd9 specifically binds the element in vitro. The crystal structure of Rmd9 bound to its dodecamer target reveals that Rmd9 belongs to the family of pentatricopeptide (PPR) proteins and uses a previously unobserved mode of specific RNA recognition. Rmd9 protects RNA from degradation by the mitochondrial 3′-exoribonuclease complex mtEXO in vitro, indicating that recognition and binding of the dodecamer element by Rmd9 confers stability to yeast mitochondrial mRNAs. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Gene expression; Mitochondria; PAR-CLIP; PPR proteins; Protein-RNA complex
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179885
作者单位	Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, 37077, Germany; Department of Cellular Biochemistry, University Medical Center Göttingen, Göttingen, 37073, Germany; Department of Cell Biology and Neuroscience, Rowan University, School of Osteopathic Medicine, Stratford, NJ 08084, United States; Department of Biochemistry and Molecular Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, United States; Department of Human Resource Information Systems, Thomas Jefferson University and Jefferson Health, Philadelphia, PA 19107, United States
推荐引用方式 GB/T 7714	Hillen H.S.,Markov D.A.,Wojtas I.D.,等. The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3′-UTRs of yeast mitochondrial mRNAs[J],2021,118(15).
APA	Hillen H.S..,Markov D.A..,Wojtas I.D..,Hofmann K.B..,Lidschreiber M..,...&Anikin M..(2021).The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3′-UTRs of yeast mitochondrial mRNAs.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Hillen H.S.,et al."The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3′-UTRs of yeast mitochondrial mRNAs".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).