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DOI	10.1073/pnas.2001897118
	Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success
	Immarigeon C.; Frei Y.; Delbare S.Y.N.; Gligorov D.; Almeida P.M.; Grey J.; Fabbro L.; Nagoshi E.; Billeter J.-C.; Wolfner M.F.; Karch F.; Maeda R.K.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Even in well-characterized genomes, many transcripts are considered noncoding RNAs (ncRNAs) simply due to the absence of large open reading frames (ORFs). However, it is now becoming clear that many small ORFs (smORFs) produce peptides with important biological functions. In the process of characterizing the ribosome-bound transcriptome of an important cell type of the seminal fluid-producing accessory gland of Drosophila melanogaster, we detected an RNA, previously thought to be noncoding, called male-specific abdominal (msa). Notably, msa is nested in the HOX gene cluster of the Bithorax complex and is known to contain a micro-RNA within one of its introns. We find that this RNA encodes a “micropeptide” (9 or 20 amino acids, MSAmiP) that is expressed exclusively in the secondary cells of the male accessory gland, where it seems to accumulate in nuclei. Importantly, loss of function of this micropeptide causes defects in sperm competition. In addition to bringing insights into the biology of a rare cell type, this work underlines the importance of small peptides, a class of molecules that is now emerging as important actors in complex biological processes. © This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Accessory gland; Drosophila; Postmating response; Reproduction; SmORF peptide
语种	英语
scopus关键词	green fluorescent protein; Hox protein; microRNA; peptide; transcriptome; untranslated RNA; accessory sex gland; amino acid sequence; animal cell; Article; cell nucleus; cells by body anatomy; controlled study; cytology; Drosophila melanogaster; Hox gene; intron; male; nonhuman; open reading frame; peptide analysis; peptide synthesis; priority journal; protein function; real time reverse transcription polymerase chain reaction; reproductive success; ribosome; RNA analysis; seminal plasma
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179874
作者单位	Department of Genetics and Evolution Sciences III, University of Geneva, Geneva 4, 1211, Switzerland; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703, United States; Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, 9700 CC, Netherlands; Molecular, Cellular and Developmental Biology (MCD) Unit, Centre de Biologie Intégrative, Université de Toulouse, CNRS, Bat 4R4, Toulouse, F-31062, France
推荐引用方式 GB/T 7714	Immarigeon C.,Frei Y.,Delbare S.Y.N.,et al. Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success[J],2021,118(15).
APA	Immarigeon C..,Frei Y..,Delbare S.Y.N..,Gligorov D..,Almeida P.M..,...&Maeda R.K..(2021).Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Immarigeon C.,et al."Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).