气候变化领域集成服务门户(CCPortal): Capture and metabolomic analysis of the human endometrial epithelial organoid secretome

CCPortal

DOI	10.1073/pnas.2026804118
	Capture and metabolomic analysis of the human endometrial epithelial organoid secretome
	Simintiras C.A.; Dhakal P.; Ranjit C.; Fitzgerald H.C.; Balboula A.Z.; Spencer T.E.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	Suboptimal uterine fluid (UF) composition can lead to pregnancy loss and likely contributes to offspring susceptibility to chronic adult-onset disorders. However, our understanding of the biochemical composition and mechanisms underpinning UF formation and regulation remain elusive, particularly in humans. To address this challenge, we developed a high-throughput method for intraorganoid fluid (IOF) isolation from human endometrial epithelial organoids. The IOF is biochemically distinct to the extraorganoid fluid (EOF) and cell culture medium as evidenced by the exclusive presence of 17 metabolites in IOF. Similarly, 69 metabolites were unique to EOF, showing asymmetrical apical and basolateral secretion by the in vitro endometrial epithelium, in a manner resembling that observed in vivo. Contrasting the quantitative metabolomic profiles of IOF and EOF revealed donor-specific biochemical signatures of organoids. Subsequent RNA sequencing of these organoids from which IOF and EOF were derived established the capacity to readily perform organoid multiomics in tandem, and suggests that transcriptomic regulation underpins the observed secretory asymmetry. In summary, these data provided by modeling uterine luminal and basolateral fluid formation in vitro offer scope to better understand UF composition and regulation with potential impacts on female fertility and offspring well-being. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Endometrium; Human; Organoid; Reproduction; Uterine fluid
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179870
作者单位	Division of Animal Sciences, University of Missouri, Columbia, MO 65211, United States; Department of Obstetrics, Gynecology, and Women's Health, University of Missouri, Columbia, MO 65201, United States
推荐引用方式 GB/T 7714	Simintiras C.A.,Dhakal P.,Ranjit C.,et al. Capture and metabolomic analysis of the human endometrial epithelial organoid secretome[J],2021,118(15).
APA	Simintiras C.A.,Dhakal P.,Ranjit C.,Fitzgerald H.C.,Balboula A.Z.,&Spencer T.E..(2021).Capture and metabolomic analysis of the human endometrial epithelial organoid secretome.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Simintiras C.A.,et al."Capture and metabolomic analysis of the human endometrial epithelial organoid secretome".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).