气候变化领域集成服务门户(CCPortal): Collagen IV differentially regulates planarian stem cell potency and lineage progression

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DOI	10.1073/pnas.2021251118
	Collagen IV differentially regulates planarian stem cell potency and lineage progression
	Chan A.; Ma S.; Pearson B.J.; Chan D.
发表日期	2021
ISSN	00278424
卷号	118 期号:16
英文摘要	The extracellular matrix (ECM) provides a precise physical and molecular environment for cell maintenance, self-renewal, and differentiation in the stem cell niche. However, the nature and organization of the ECM niche is not well understood. The adult freshwater planarian Schmidtea mediterranea maintains a large population of multipotent stem cells (neoblasts), presenting an ideal model to study the role of the ECM niche in stem cell regulation. Here we tested the function of 165 planarian homologs of ECM and ECM-related genes in neoblast regulation. We identified the collagen gene family as one with differential effects in promoting or suppressing proliferation of neoblasts. col4-1, encoding a type IV collagen α-chain, had the strongest effect. RNA interference (RNAi) of col4-1 impaired tissue maintenance and regeneration, causing tissue regression. Finally, we provide evidence for an interaction between type IV collagen, the discoidin domain receptor, and neuregulin-7 (NRG-7), which constitutes a mechanism to regulate the balance of symmetric and asymmetric division of neoblasts via the NRG-7/EGFR pathway. © This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Extracellular matrix; Planarian; Pluripotency; Regeneration; Stem cell niche
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179814
作者单位	School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong, Hong Kong; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A1, Canada; Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Ontario Institute for Cancer Research, Toronto, ON M5G 0A3, Canada
推荐引用方式 GB/T 7714	Chan A.,Ma S.,Pearson B.J.,et al. Collagen IV differentially regulates planarian stem cell potency and lineage progression[J],2021,118(16).
APA	Chan A.,Ma S.,Pearson B.J.,&Chan D..(2021).Collagen IV differentially regulates planarian stem cell potency and lineage progression.Proceedings of the National Academy of Sciences of the United States of America,118(16).
MLA	Chan A.,et al."Collagen IV differentially regulates planarian stem cell potency and lineage progression".Proceedings of the National Academy of Sciences of the United States of America 118.16(2021).