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DOI	10.1073/pnas.2025658118
	Cryo-EM structure of Mycobacterium smegmatis DyP-loaded encapsulin
	Tang Y.; Mu A.; Zhang Y.; Zhou S.; Wang W.; Lai Y.; Zhou X.; Liu F.; Yang X.; Gong H.; Wang Q.; Rao Z.
发表日期	2021
ISSN	00278424
卷号	118 期号:16
英文摘要	Encapsulins containing dye-decolorizing peroxidase (DyP)-type peroxidases are ubiquitous among prokaryotes, protecting cells against oxidative stress. However, little is known about how they interact and function. Here, we have isolated a native cargo-packaging encapsulin from Mycobacterium smegmatis and determined its complete high-resolution structure by cryogenic electron microscopy (cryo-EM). This encapsulin comprises an icosahedral shell and a dodecameric DyP cargo. The dodecameric DyP consists of two hexamers with a twofold axis of symmetry and stretches across the interior of the encapsulin. Our results reveal that the encapsulin shell plays a role in stabilizing the dodecameric DyP. Furthermore, we have proposed a potential mechanism for removing the hydrogen peroxide based on the structural features. Our study also suggests that the DyP is the primary cargo protein of mycobacterial encapsulins and is a potential target for antituberculosis drug discovery. © This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	Cryo-electron microscopy; Dye-decolorizing peroxidase; Encapsulin nanocompartment; Mycobacterium smegmatis
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179803
作者单位	State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin, 300353, China; National Laboratory of Biomacromolecules, Chinese Academy of Sciences Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin, 300071, China; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China
推荐引用方式 GB/T 7714	Tang Y.,Mu A.,Zhang Y.,et al. Cryo-EM structure of Mycobacterium smegmatis DyP-loaded encapsulin[J],2021,118(16).
APA	Tang Y..,Mu A..,Zhang Y..,Zhou S..,Wang W..,...&Rao Z..(2021).Cryo-EM structure of Mycobacterium smegmatis DyP-loaded encapsulin.Proceedings of the National Academy of Sciences of the United States of America,118(16).
MLA	Tang Y.,et al."Cryo-EM structure of Mycobacterium smegmatis DyP-loaded encapsulin".Proceedings of the National Academy of Sciences of the United States of America 118.16(2021).