气候变化领域集成服务门户(CCPortal): An alternative resource allocation strategy in the chemolithoautotrophic archaeon Methanococcus maripaludis

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DOI	10.1073/pnas.2025854118
	An alternative resource allocation strategy in the chemolithoautotrophic archaeon Methanococcus maripaludis
	Müller A.L.; Gu W.; Patsalo V.; Deutzmann J.S.; Williamson J.R.; Spormann A.M.
发表日期	2021
ISSN	00278424
卷号	118 期号:16
英文摘要	Most microorganisms in nature spend the majority of time in a state of slow or zero growth and slow metabolism under limited energy or nutrient flux rather than growing at maximum rates. Yet, most of our knowledge has been derived from studies on fast-growing bacteria. Here, we systematically characterized the physiology of the methanogenic archaeon Methanococcus maripaludis during slow growth. M. maripaludis was grown in continuous culture under energy (formate)-limiting conditions at different dilution rates ranging from 0.09 to 0.002 h−1, the latter corresponding to 1% of its maximum growth rate under laboratory conditions (0.23 h−1). While the specific rate of methanogenesis correlated with growth rate as expected, the fraction of cellular energy used for maintenance increased and the maintenance energy per biomass decreased at slower growth. Notably, proteome allocation between catabolic and anabolic pathways was invariant with growth rate. Unexpectedly, cells maintained their maximum methanogenesis capacity over a wide range of growth rates, except for the lowest rates tested. Cell size, cellular DNA, RNA, and protein content as well as ribosome numbers also were largely invariant with growth rate. A reduced protein synthesis rate during slow growth was achieved by a reduction in ribosome activity rather than via the number of cellular ribosomes. Our data revealed a resource allocation strategy of a methanogenic archaeon during energy limitation that is fundamentally different from commonly studied versatile chemoheterotrophic bacteria such as E. coli. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Maintenance energy; Methanogen; Proteome allocation; Ribosome activity; Slow growth
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179790
作者单位	Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305, United States; Department of Integrative Structural and Computational Biology, Department of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, San Diego, CA 92037, United States
推荐引用方式 GB/T 7714	Müller A.L.,Gu W.,Patsalo V.,et al. An alternative resource allocation strategy in the chemolithoautotrophic archaeon Methanococcus maripaludis[J],2021,118(16).
APA	Müller A.L.,Gu W.,Patsalo V.,Deutzmann J.S.,Williamson J.R.,&Spormann A.M..(2021).An alternative resource allocation strategy in the chemolithoautotrophic archaeon Methanococcus maripaludis.Proceedings of the National Academy of Sciences of the United States of America,118(16).
MLA	Müller A.L.,et al."An alternative resource allocation strategy in the chemolithoautotrophic archaeon Methanococcus maripaludis".Proceedings of the National Academy of Sciences of the United States of America 118.16(2021).