气候变化领域集成服务门户(CCPortal): Human retroviral antisense mRNAs are retained in the nuclei of infected cells for viral persistence

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DOI	10.1073/pnas.2014783118
	Human retroviral antisense mRNAs are retained in the nuclei of infected cells for viral persistence
	Ma G.; Yasunaga J.-I.; Shimura K.; Takemoto K.; Watanabe M.; Amano M.; Nakata H.; Liu B.; Zuo X.; Matsuoka M.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Human retroviruses, including human T cell leukemia virus type 1 (HTLV-1) and HIV type 1 (HIV-1), encode an antisense gene in the negative strand of the provirus. Besides coding for proteins, the messenger RNAs (mRNAs) of retroviral antisense genes have also been found to regulate transcription directly. Thus, it has been proposed that retroviruses likely localize their antisense mRNAs to the nucleus in order to regulate nuclear events; however, this opposes the coding function of retroviral antisense mRNAs that requires a cytoplasmic localization for protein translation. Here, we provide direct evidence that retroviral antisense mRNAs are localized predominantly in the nuclei of infected cells. The retroviral 3′ LTR induces inefficient polyadenylation and nuclear retention of antisense mRNA. We further reveal that retroviral antisense RNAs retained in the nucleus associate with chromatin and have transcriptional regulatory function. While HTLV-1 antisense mRNA is recruited to the promoter of C-C chemokine receptor type 4 (CCR4) and enhances transcription from it to support the proliferation of HTLV-1–infected cells, HIV-1 antisense mRNA is recruited to the viral LTR and inhibits sense mRNA expression to maintain the latency of HIV-1 infection. In summary, retroviral antisense mRNAs are retained in nucleus, act like long noncoding RNAs instead of mRNAs, and contribute to viral persistence. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	ASP; HBZ; HIV-1; HTLV-1; RNA-FISH
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179775
作者单位	Institute of Pharmaceutical Science, China Pharmaceutical University, Nanjing, 210009, China; Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, 606-8507, Japan; Department of Hematology, Rheumatology and Infectious Diseases, Kumamoto University School of Medicine, Kumamoto, 860-8556, Japan
推荐引用方式 GB/T 7714	Ma G.,Yasunaga J.-I.,Shimura K.,et al. Human retroviral antisense mRNAs are retained in the nuclei of infected cells for viral persistence[J],2021,118(17).
APA	Ma G..,Yasunaga J.-I..,Shimura K..,Takemoto K..,Watanabe M..,...&Matsuoka M..(2021).Human retroviral antisense mRNAs are retained in the nuclei of infected cells for viral persistence.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Ma G.,et al."Human retroviral antisense mRNAs are retained in the nuclei of infected cells for viral persistence".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).