气候变化领域集成服务门户(CCPortal): Incipient genome erosion and metabolic streamlining for antibiotic production in a defensive symbiont

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DOI	10.1073/pnas.2023047118
	Incipient genome erosion and metabolic streamlining for antibiotic production in a defensive symbiont
	Nechitaylo T.Y.; Sandoval-Calderón M.; Engl T.; Wielsch N.; Dunn D.M.; Goesmann A.; Strohm E.; Svatoš A.; Dale C.; Weiss R.B.; Kaltenpoth M.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Genome erosion is a frequently observed result of relaxed selection in insect nutritional symbionts, but it has rarely been studied in defensive mutualisms. Solitary beewolf wasps harbor an actinobacterial symbiont of the genus Streptomyces that provides protection to the developing offspring against pathogenic microorganisms. Here, we characterized the genomic architecture and functional gene content of this culturable symbiont using genomics, transcriptomics, and proteomics in combination with in vitro assays. Despite retaining a large linear chromosome (7.3 Mb), the wasp symbiont accumulated frameshift mutations in more than a third of its protein-coding genes, indicative of incipient genome erosion. Although many of the frameshifted genes were still expressed, the encoded proteins were not detected, indicating post-transcriptional regulation. Most pseudogenization events affected accessory genes, regulators, and transporters, but “Streptomyces philanthi” also experienced mutations in central metabolic pathways, resulting in auxotrophies for biotin, proline, and arginine that were confirmed experimentally in axenic culture. In contrast to the strong A+T bias in the genomes of most obligate symbionts, we observed a significant G+C enrichment in regions likely experiencing reduced selection. Differential expression analyses revealed that—compared to in vitro symbiont cultures—“S. philanthi” in beewolf antennae showed overexpression of genes for antibiotic biosynthesis, the uptake of host-provided nutrients and the metabolism of building blocks required for antibiotic production. Our results show unusual traits in the early stage of genome erosion in a defensive symbiont and suggest tight integration of host–symbiont metabolic pathways that effectively grants the host control over the antimicrobial activity of its bacterial partner. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Defensive symbiosis; Genome erosion; Protective mutualism; Pseudogenization; Streptomyces
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179773
作者单位	Research Group Insect Symbiosis, Max Planck Institute for Chemical Ecology, Jena, 07745, Germany; Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg University, Mainz, 55128, Germany; Department of Insect Symbiosis, Max Planck Institute for Chemical Ecology, Jena, 07745, Germany; Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, Jena, 07745, Germany; Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, United States; Bioinformatics and Systems Biology, Justus Liebig University Gießen, Gießen, 35390, Germany; Department of Zoology, University of Regensburg, Regensburg, 93053, Germany; School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, United States
推荐引用方式 GB/T 7714	Nechitaylo T.Y.,Sandoval-Calderón M.,Engl T.,et al. Incipient genome erosion and metabolic streamlining for antibiotic production in a defensive symbiont[J],2021,118(17).
APA	Nechitaylo T.Y..,Sandoval-Calderón M..,Engl T..,Wielsch N..,Dunn D.M..,...&Kaltenpoth M..(2021).Incipient genome erosion and metabolic streamlining for antibiotic production in a defensive symbiont.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Nechitaylo T.Y.,et al."Incipient genome erosion and metabolic streamlining for antibiotic production in a defensive symbiont".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).