气候变化领域集成服务门户(CCPortal): Small noncoding RNA profiling across cellular and biofluid compartments and their implications for multiple sclerosis immunopathology

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DOI	10.1073/pnas.2011574118
	Small noncoding RNA profiling across cellular and biofluid compartments and their implications for multiple sclerosis immunopathology
	Zheleznyakova G.Y.; Piket E.; Needhamsen M.; Hagemann-Jensen M.; Ekman D.; Han Y.; James T.; Khademi M.; Al Nimer F.; Scicluna P.; Huang J.; Kockum I.; Faridani O.R.; Olsson T.; Piehl F.; Jagodic M.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease affecting the central nervous system (CNS). Small noncoding RNAs (sncRNAs) and, in particular, microRNAs (miRNAs) have frequently been associated with MS. Here, we performed a comprehensive analysis of all classes of sncRNAs in matching samples of peripheral blood mononuclear cells (PBMCs), plasma, cerebrospinal fluid (CSF) cells, and cell-free CSF from relapsing-remitting (RRMS, n = 12 in relapse and n = 11 in remission) patients, secondary progressive (SPMS, n = 6) MS patients, and noninflammatory and inflammatory neurological disease controls (NINDC, n = 11; INDC, n = 5). We show widespread changes in miRNAs and sncRNA-derived fragments of small nuclear, nucleolar, and transfer RNAs. In CSF cells, 133 out of 133 and 115 out of 117 differentially expressed sncRNAs were increased in RRMS relapse compared to remission and RRMS compared to NINDC, respectively. In contrast, 65 out of 67 differentially expressed PBMC sncRNAs were decreased in RRMS compared to NINDC. The striking contrast between the periphery and CNS suggests that sncRNA-mediated mechanisms, including alternative splicing, RNA degradation, and mRNA translation, regulate the transcriptome of pathogenic cells primarily in the CNS target organ. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Micrornas; Multiple sclerosis; Small noncoding RNAs
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179741
作者单位	Department of Clinical Neuroscience, Karolinska Institutet, Center for Molecular Medicine, Karolinska University Hospital, Stockholm, SE-171 76, Sweden; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, SE-171 77, Sweden; Department of Biochemistry and Biophysics, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Stockholm University, Stockholm, SE-171 21, Sweden; Department of Oncology-Pathology, Science for Life Laboratory, Karolinska Institutet, Stockholm, SE-171 21, Sweden; Institute for Molecular Medicine Finland, Helsinki Institute of Life Science, University of Helsinki, Helsinki, FI-00290, Finland; Center of Neurology, Academic Specialist Center, Stockholm Health Services, Stockholm, SE-113 65, Sweden; Lowy Cancer Research Centre, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Garvan Institute of Medical Research, Sydney, NSW 2010, Australia
推荐引用方式 GB/T 7714	Zheleznyakova G.Y.,Piket E.,Needhamsen M.,et al. Small noncoding RNA profiling across cellular and biofluid compartments and their implications for multiple sclerosis immunopathology[J],2021,118(17).
APA	Zheleznyakova G.Y..,Piket E..,Needhamsen M..,Hagemann-Jensen M..,Ekman D..,...&Jagodic M..(2021).Small noncoding RNA profiling across cellular and biofluid compartments and their implications for multiple sclerosis immunopathology.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Zheleznyakova G.Y.,et al."Small noncoding RNA profiling across cellular and biofluid compartments and their implications for multiple sclerosis immunopathology".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).