气候变化领域集成服务门户(CCPortal): An introgressed gene causes meiotic drive in Neurospora sitophila

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DOI	10.1073/pnas.2026605118
	An introgressed gene causes meiotic drive in Neurospora sitophila
	Svedberg J.; Vogan A.A.; Rhoades N.A.; Sarmarajeewa D.; Jacobson D.J.; Lascoux M.; Hammond T.M.; Johannesson H.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Meiotic drive elements cause their own preferential transmission following meiosis. In fungi, this phenomenon takes the shape of spore killing, and in the filamentous ascomycete Neurospora sitophila, the Sk-1 spore killer element is found in many natural populations. In this study, we identify the gene responsible for spore killing in Sk-1 by generating both long- and short-read genomic data and by using these data to perform a genome-wide association test. We name this gene Spk-1. Through molecular dissection, we show that a single 405-nt-long open reading frame generates a product that both acts as a poison capable of killing sibling spores and as an antidote that rescues spores that produce it. By phylogenetic analysis, we demonstrate that the gene has likely been introgressed from the closely related species Neurospora hispaniola, and we identify three subclades of N. sitophila, one where Sk-1 is fixed, another where Sk-1 is absent, and a third where both killer and sensitive strain are found. Finally, we show that spore killing can be suppressed through an RNA interference-based genome defense pathway known as meiotic silencing by unpaired DNA. Spk-1 is not related to other known meiotic drive genes, and similar sequences are only found within Neurospora. These results shed light on the diversity of genes capable of causing meiotic drive, their origin and evolution, and their interaction with the host genome. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Genomic conflict; Meiotic drive; Neurospora; Spore killer
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179740
作者单位	Department of Organismal Biology, Evolutionary Biology Centre (EBC), Uppsala University, Uppsala, 752 36, Sweden; Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, United States; School of Biological Sciences, Illinois State University, Normal, IL 61790, United States; Department of Ecology and Genetics, EBC, Uppsala University, Uppsala, 752 36, Sweden
推荐引用方式 GB/T 7714	Svedberg J.,Vogan A.A.,Rhoades N.A.,et al. An introgressed gene causes meiotic drive in Neurospora sitophila[J],2021,118(17).
APA	Svedberg J..,Vogan A.A..,Rhoades N.A..,Sarmarajeewa D..,Jacobson D.J..,...&Johannesson H..(2021).An introgressed gene causes meiotic drive in Neurospora sitophila.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Svedberg J.,et al."An introgressed gene causes meiotic drive in Neurospora sitophila".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).