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DOI	10.1073/pnas.2022464118
	A pocket-factor–triggered conformational switch in the hepatitis B virus capsid
	Lecoq L.; Wang S.; Dujardin M.; Zimmermann P.; Schuster L.; Fogeron M.-L.; Briday M.; Schledorn M.; Wiegand T.; Cole L.; Montserret R.; Bressanelli S.; Meier B.H.; Nassal M.; Böckmann A.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Viral hepatitis is growing into an epidemic illness, and it is urgent to neutralize the main culprit, hepatitis B virus (HBV), a small-enveloped retrotranscribing DNA virus. An intriguing observation in HB virion morphogenesis is that capsids with immature genomes are rarely enveloped and secreted. This prompted, in 1982, the postulate that a regulated conformation switch in the capsid triggers envelopment. Using solid-state NMR, we identified a stable alternative conformation of the capsid. The structural variations focus on the hydrophobic pocket of the core protein, a hot spot in capsid–envelope interactions. This structural switch is triggered by specific, high-affinity binding of a pocket factor. The conformational change induced by the binding is reminiscent of a maturation signal. This leads us to formulate the “synergistic double interaction” hypothesis, which explains the regulation of capsid envelopment and indicates a concept for therapeutic interference with HBV envelopment. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Hepatitis B virus; Hydrophobic pocket; Solid-state NMR; Triton
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179735
作者单位	Molecular Microbiology and Structural Biochemistry, Labex Ecofect, UMR 5086 CNRS/Université de Lyon, Lyon, 69367, France; Department of Medicine II/Molecular Biology, University Hospital Freiburg, Medical Center, University of Freiburg, Freiburg, 79106, Germany; Physical Chemistry, ETH Zurich, Zurich, 8093, Switzerland; Institute for Integrative Biology of the Cell, Commissariat à l’énergie Atomique, CNRS, Université Paris-Saclay, Gif sur Yvette Cedex, 91198, France
推荐引用方式 GB/T 7714	Lecoq L.,Wang S.,Dujardin M.,等. A pocket-factor–triggered conformational switch in the hepatitis B virus capsid[J],2021,118(17).
APA	Lecoq L..,Wang S..,Dujardin M..,Zimmermann P..,Schuster L..,...&Böckmann A..(2021).A pocket-factor–triggered conformational switch in the hepatitis B virus capsid.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Lecoq L.,et al."A pocket-factor–triggered conformational switch in the hepatitis B virus capsid".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).