气候变化领域集成服务门户(CCPortal): Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating

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DOI	10.1073/pnas.2015689118
	Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating
	Zhao L.; Xie H.; Kang Y.; Lin Y.; Liu G.; Sakato-Antoku M.; Patel-King R.S.; Wang B.; Wan C.; King S.M.; Zhao C.; Huang K.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Coordinated beating is crucial for the function of multiple cilia. However, the molecular mechanism is poorly understood. Here, we characterize a conserved ciliary protein CYB5D1 with a heme-binding domain and a cordon-bleu ubiquitin-like domain. Mutation or knockdown of Cyb5d1 in zebrafish impaired coordinated ciliary beating in the otic vesicle and olfactory epithelium. Similarly, the two flagella of an insertional mutant of the CYB5D1 ortholog in Chlamydomonas (Crcyb5d1) showed an uncoordinated pattern due to a defect in the cis-flagellum. Biochemical analyses revealed that CrCYB5D1 is a radial spoke stalk protein that binds heme only under oxidizing conditions. Lack of CrCYB5D1 resulted in a reductive shift in flagellar redox state and slowing down of the phototactic response. Treatment of Crcyb5d1 with oxidants restored coordinated flagellar beating. Taken together, these data suggest that CrCYB5D1 may integrate environmental and intraciliary signals and regulate the redox state of cilia, which is crucial for the coordinated beating of multiple cilia. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Chlamydomonas; Cilia; Motility; Redox; Zebrafish
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179724
作者单位	Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100039, China; Institute of Evolution and Marine Biodiversity, College of Marine Life Science, Ocean University of China, Qingdao, 266003, China; Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 06032-3305, United States; Hubei Key Lab of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266003, China
推荐引用方式 GB/T 7714	Zhao L.,Xie H.,Kang Y.,et al. Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating[J],2021,118(17).
APA	Zhao L..,Xie H..,Kang Y..,Lin Y..,Liu G..,...&Huang K..(2021).Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Zhao L.,et al."Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).