气候变化领域集成服务门户(CCPortal): An ABC transporter Wzm–Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria

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DOI	10.1073/pnas.2023663118
	An ABC transporter Wzm–Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria
	Savková K.; Huszár S.; Baráth P.; Pakanová Z.; Kozmon S.; Vancová M.; Tesarová M.; Blaško J.; Kalinák M.; Singh V.; Korduláková J.; Mikušová K.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Mycobacterium tuberculosis, one of the deadliest pathogens in human history, is distinguished by a unique, multilayered cell wall, which offers the bacterium a high level of protection from the attacks of the host immune system. The primary structure of the cell wall core, composed of covalently linked peptidoglycan, branched heteropolysaccharide arabinogalactan, and mycolic acids, is well known, and numerous enzymes involved in the biosynthesis of its components are characterized. The cell wall biogenesis takes place at both cytoplasmic and periplasmic faces of the plasma membrane, and only recently some of the specific transport systems translocating the metabolic intermediates between these two compartments have been characterized [M. Jackson, C. M. Stevens, L. Zhang, H. I. Zgurskaya, M. Niederweis, Chem. Rev., 10.1021/acs.chemrev.0c00869 (2020)]. In this work, we use CRISPR interference methodology in Mycobacterium smegmatis to functionally characterize an ATP-binding cassette (ABC) transporter involved in the translocation of galactan precursors across the plasma membrane. We show that genetic knockdown of the transmembrane subunit of the transporter results in severe morphological changes and the accumulation of an aberrantly long galactan precursor. Based on similarities with structures and functions of specific O-antigen ABC transporters of gram-negative bacteria [C. Whitfield, D. M. Williams, S. D. Kelly, J. Biol. Chem. 295, 10593-10609 (2020)], we propose a model for coupled synthesis and export of the galactan polymer precursor in mycobacteria. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell wall; Galactan; Mycobacterium; Transport
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179713
作者单位	Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, 842 15, Slovakia; Institute of Chemistry, Slovak Academy of Sciences, Bratislava, 845 38, Slovakia; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceské Budejovice, 370 05, Czech Republic; Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, 842 15, Slovakia; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, 812 37, Slovakia; South African Medical Research Council, Drug Discovery and Development Research Unit, Department of Chemistry, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Rondebosch, 7701, South Africa; Drug Discovery and Development Centre, University of Cape Town, Rondebosch, 7701, South Africa
推荐引用方式 GB/T 7714	Savková K.,Huszár S.,Baráth P.,等. An ABC transporter Wzm–Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria[J],2021,118(17).
APA	Savková K..,Huszár S..,Baráth P..,Pakanová Z..,Kozmon S..,...&Mikušová K..(2021).An ABC transporter Wzm–Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Savková K.,et al."An ABC transporter Wzm–Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).