气候变化领域集成服务门户(CCPortal): Shortened tethering filaments stabilize presynaptic vesicles in support of elevated release probability during LTP in rat hippocampus

CCPortal

DOI	10.1073/pnas.2018653118
	Shortened tethering filaments stabilize presynaptic vesicles in support of elevated release probability during LTP in rat hippocampus
	Jung J.H.; Kirk L.M.; Bourne J.N.; Harris K.M.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Long-term potentiation (LTP) is a cellular mechanism of learning and memory that results in a sustained increase in the probability of vesicular release of neurotransmitter. However, previous work in hippocampal area CA1 of the adult rat revealed that the total number of vesicles per synapse decreases following LTP, seemingly inconsistent with the elevated release probability. Here, electron-microscopic tomography (EMT) was used to assess whether changes in vesicle density or structure of vesicle tethering filaments at the active zone might explain the enhanced release probability following LTP. The spatial relationship of vesicles to the active zone varies with functional status. Tightly docked vesicles contact the presynaptic membrane, have partially formed SNARE complexes, and are primed for release of neurotransmitter upon the next action potential. Loosely docked vesicles are located within 8 nm of the presynaptic membrane where SNARE complexes begin to form. Nondocked vesicles comprise recycling and reserve pools. Vesicles are tethered to the active zone via filaments composed of molecules engaged in docking and release processes. The density of tightly docked vesicles was increased 2 h following LTP compared to control stimulation, whereas the densities of loosely docked or nondocked vesicles congregating within 45 nm above the active zones were unchanged. The tethering filaments on all vesicles were shorter and their attachment sites shifted closer to the active zone. These findings suggest that tethering filaments stabilize more vesicles in the primed state. Such changes would facilitate the long-lasting increase in release probability following LTP. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Long-term potentiation; Nanoscale; Synaptic plasticity; Ultrastructure
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179712
作者单位	Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, United States; Department of Biology, Texas A&M University, College Station, TX 77843, United States; Department of Neuroscience, Center for Learning and Memory, Institute for Neuroscience, University of Texas at Austin, Austin, TX 78712, United States; Department of Cell and Developmental Biology, University of Colorado, Aurora, CO 80045, United States
推荐引用方式 GB/T 7714	Jung J.H.,Kirk L.M.,Bourne J.N.,et al. Shortened tethering filaments stabilize presynaptic vesicles in support of elevated release probability during LTP in rat hippocampus[J],2021,118(17).
APA	Jung J.H.,Kirk L.M.,Bourne J.N.,&Harris K.M..(2021).Shortened tethering filaments stabilize presynaptic vesicles in support of elevated release probability during LTP in rat hippocampus.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Jung J.H.,et al."Shortened tethering filaments stabilize presynaptic vesicles in support of elevated release probability during LTP in rat hippocampus".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).