气候变化领域集成服务门户(CCPortal): Blood-brain barrier opening by intracarotid artery hyperosmolar mannitol induces sterile inflammatory and innate immune responses

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DOI	10.1073/pnas.2021915118
	Blood-brain barrier opening by intracarotid artery hyperosmolar mannitol induces sterile inflammatory and innate immune responses
	Burks S.R.; Kersch C.N.; Witko J.A.; Pagel M.A.; Sundby M.; Muldoon L.L.; Neuwelt E.A.; Frank J.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:18
英文摘要	Intracarotid arterial hyperosmolar mannitol (ICAHM) blood-brain barrier disruption (BBBD) is effective and safe for delivery of therapeutics for central nervous system malignancies. ICAHM osmotically alters endothelial cells and tight junction integrity to achieve BBBD. However, occurrence of neuroinflammation following hemispheric BBBD by ICAHM remains unknown. Temporal proteomic changes in rat brains following ICAHM included increased damage-associated molecular patterns, cytokines, chemokines, trophic factors, and cell adhesion molecules, indicative of a sterile inflammatory response (SIR). Proteomic changes occurred within 5 min of ICAHM infusion and returned to baseline by 96 h. Transcriptomic analyses following ICAHM BBBD further supported an SIR. Immunohistochemistry revealed activated astrocytes, microglia, and macrophages. Moreover, proinflammatory proteins were elevated in serum, and proteomic and histological findings from the contralateral hemisphere demonstrated a less pronounced SIR, suggesting neuroinflammation beyond regions of ICAHM infusion. Collectively, these results demonstrate ICAHM induces a transient SIR that could potentially be harnessed for neuroimmunomodulation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Blood-brain barrier; Hyperosmolar mannitol; Neuroinflammation; Sterile inflammation
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179702
作者单位	Frank Laboratory, Radiology and Imaging Sciences, NIH Clinical Center, Bethesda, MD 20895, United States; Department of Neurology, Oregon Health & Science University, Portland, OR 97239, United States; Department of Neurological Surgery, Oregon Health & Science University, Portland, OR 97239, United States; Portland Veterans Affairs Medical Center, Portland, OR 97239, United States; National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD 20895, United States
推荐引用方式 GB/T 7714	Burks S.R.,Kersch C.N.,Witko J.A.,et al. Blood-brain barrier opening by intracarotid artery hyperosmolar mannitol induces sterile inflammatory and innate immune responses[J],2021,118(18).
APA	Burks S.R..,Kersch C.N..,Witko J.A..,Pagel M.A..,Sundby M..,...&Frank J.A..(2021).Blood-brain barrier opening by intracarotid artery hyperosmolar mannitol induces sterile inflammatory and innate immune responses.Proceedings of the National Academy of Sciences of the United States of America,118(18).
MLA	Burks S.R.,et al."Blood-brain barrier opening by intracarotid artery hyperosmolar mannitol induces sterile inflammatory and innate immune responses".Proceedings of the National Academy of Sciences of the United States of America 118.18(2021).