气候变化领域集成服务门户(CCPortal): Anaerobic gut fungi are an untapped reservoir of natural products

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DOI	10.1073/pnas.2019855118
	Anaerobic gut fungi are an untapped reservoir of natural products
	Swift C.L.; Louie K.B.; Bowen B.P.; Olson H.M.; Purvine S.O.; Salamov A.; Mondo S.J.; Solomon K.V.; Wright A.T.; Northen T.R.; Grigoriev I.V.; Keller N.P.; O'Malley M.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:18
英文摘要	Anaerobic fungi (class Neocallimastigomycetes) thrive as low-abundance members of the herbivore digestive tract. The genomes of anaerobic gut fungi are poorly characterized and have not been extensively mined for the biosynthetic enzymes of natural products such as antibiotics. Here, we investigate the potential of anaerobic gut fungi to synthesize natural products that could regulate membership within the gut microbiome. Complementary'omics' approaches were combined to catalog the natural products of anaerobic gut fungi from four different representative species: Anaeromyces robustus (A. robustus), Caecomyces churrovis (C. churrovis), Neocallimastix californiae (N. californiae), and Piromyces finnis (P. finnis). In total, 146 genes were identified that encode biosynthetic enzymes for diverse types of natural products, including nonribosomal peptide synthetases and polyketide synthases. In addition, N. californiae and C. churrovis genomes encoded seven putative bacteriocins, a class of antimicrobial peptides typically produced by bacteria. During standard laboratory growth on plant biomass or soluble substrates, 26% of total core biosynthetic genes in all four strains were transcribed. Across all four fungal strains, 30% of total biosynthetic gene products were detected via proteomics when grown on cellobiose. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) characterization of fungal supernatants detected 72 likely natural products from A. robustus alone. A compound produced by all four strains of anaerobic fungi was putatively identified as the polyketide-related styrylpyrone baumin. Molecular networking quantified similarities between tandem mass spectrometry (MS/ MS) spectra among these fungi, enabling three groups of natural products to be identified that are unique to anaerobic fungi. Overall, these results support the finding that anaerobic gut fungi synthesize natural products, which could be harnessed as a source of antimicrobials, therapeutics, and other bioactive compounds. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Anaerobes; Fungi; Natural products; Secondary metabolism; Transcriptomics
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179696
作者单位	Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, United States; Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Earth and Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, United States; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, United States; Department of Agricultural Biology, Colorado State University, Fort Collins, CO 80523, United States; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, United States; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Joint BioEnergy Institute, Emeryville, CA 94608, United States; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; Department of Medical Microbiology and Immun...
推荐引用方式 GB/T 7714	Swift C.L.,Louie K.B.,Bowen B.P.,et al. Anaerobic gut fungi are an untapped reservoir of natural products[J],2021,118(18).
APA	Swift C.L..,Louie K.B..,Bowen B.P..,Olson H.M..,Purvine S.O..,...&O'Malley M.A..(2021).Anaerobic gut fungi are an untapped reservoir of natural products.Proceedings of the National Academy of Sciences of the United States of America,118(18).
MLA	Swift C.L.,et al."Anaerobic gut fungi are an untapped reservoir of natural products".Proceedings of the National Academy of Sciences of the United States of America 118.18(2021).