气候变化领域集成服务门户(CCPortal): Plant stem-cell organization and differentiation at single-cell resolution

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DOI	10.1073/PNAS.2018788117
	Plant stem-cell organization and differentiation at single-cell resolution
	Satterlee J.W.; Strable J.; Scanlon M.J.
发表日期	2021
ISSN	00278424
起始页码	33689
结束页码	33699
卷号	117 期号:52
英文摘要	Plants maintain populations of pluripotent stem cells in shoot apical meristems (SAMs), which continuously produce new aboveground organs. We used single-cell RNA sequencing (scRNA-seq) to achieve an unbiased characterization of the transcriptional landscape of the maize shoot stem-cell niche and its differentiating cellular descendants. Stem cells housed in the SAM tip are engaged in genome integrity maintenance and exhibit a low rate of cell division, consistent with their contributions to germline and somatic cell fates. Surprisingly, we find no evidence for a canonical stem-cell organizing center subtending these cells. In addition, trajectory inference was used to trace the gene expression changes that accompany cell differentiation, revealing that ectopic expression of KNOTTED1 (KN1) accelerates cell differentiation and promotes development of the sheathing maize leaf base. These single-cell transcriptomic analyses of the shoot apex yield insight into the processes of stem-cell function and cell-fate acquisition in the maize seedling and provide a valuable scaffold on which to better dissect the genetic control of plant shoot morphogenesis at the cellular level. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Cell differentiation; Maize; Shoot apical meristem; Single-cell transcriptomics
语种	英语
scopus关键词	apical meristem; article; cell culture; cell division; cell fate; cell function; ectopic expression; genetic transcription; germ line; human cell; maize; morphogenesis; nonhuman; plant leaf; plant stem; progeny; seedling; shoot; single cell RNA seq; somatic cell; stem cell niche; cell differentiation; cytology; gene expression regulation; genetics; maize; meristem; metabolism; plant genome; single cell analysis; stem cell; plant protein; transcriptome; Cell Differentiation; Cell Division; Gene Expression Regulation, Plant; Genome, Plant; Meristem; Plant Leaves; Plant Proteins; Single-Cell Analysis; Stem Cells; Transcription, Genetic; Transcriptome; Zea mays
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179660
作者单位	School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, United States
推荐引用方式 GB/T 7714	Satterlee J.W.,Strable J.,Scanlon M.J.. Plant stem-cell organization and differentiation at single-cell resolution[J],2021,117(52).
APA	Satterlee J.W.,Strable J.,&Scanlon M.J..(2021).Plant stem-cell organization and differentiation at single-cell resolution.Proceedings of the National Academy of Sciences of the United States of America,117(52).
MLA	Satterlee J.W.,et al."Plant stem-cell organization and differentiation at single-cell resolution".Proceedings of the National Academy of Sciences of the United States of America 117.52(2021).