气候变化领域集成服务门户(CCPortal): An in vivo atlas of host–pathogen transcriptomes during Streptococcus pneumoniae colonization and disease

CCPortal

DOI	10.1073/PNAS.2010428117
	An in vivo atlas of host–pathogen transcriptomes during Streptococcus pneumoniae colonization and disease
	D’Mello A.; Riegler A.N.; Martínez E.; Beno S.M.; Ricketts T.D.; Foxman E.F.; Orihuela C.J.; Tettelin H.
发表日期	2021
ISSN	00278424
起始页码	33507
结束页码	33518
卷号	117 期号:52
英文摘要	Streptococcus pneumoniae (Spn) colonizes the nasopharynx and can cause pneumonia. From the lungs it spreads to the bloodstream and causes organ damage. We characterized the in vivo Spn and mouse transcriptomes within the nasopharynx, lungs, blood, heart, and kidneys using three Spn strains. We identified Spn genes highly expressed at all anatomical sites and in an organ-specific manner; highly expressed genes were shown to have vital roles with knockout mutants. The in vivo bacterial transcriptome during colonization/disease was distinct from previously reported in vitro transcriptomes. Distinct Spn and host gene-expression profiles were observed during colonization and disease states, revealing specific genes/operons whereby Spn adapts to and influences host sites in vivo. We identified and experimentally verified host-defense pathways induced by Spn during invasive disease, including proinflammatory responses and the interferon response. These results shed light on the pathogenesis of Spn and identify therapeutic targets. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Dual species RNA-seq; Host–pathogen interactions; In vivo transcriptomics; Mouse models of colonization and invasive disease; Streptococcus pneumoniae
语种	英语
scopus关键词	interferon; transcriptome; animal; bacterial count; bacterial gene; C57BL mouse; female; gene expression regulation; genetics; growth, development and aging; host pathogen interaction; male; metabolism; microbiology; mutation; phylogeny; physiology; pneumococcal infection; principal component analysis; signal transduction; Streptococcus pneumoniae; Animals; Colony Count, Microbial; Female; Gene Expression Regulation, Bacterial; Genes, Bacterial; Host-Pathogen Interactions; Interferons; Male; Mice, Inbred C57BL; Mutation; Phylogeny; Pneumococcal Infections; Principal Component Analysis; Signal Transduction; Streptococcus pneumoniae; Transcriptome
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179632
作者单位	Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, United States; Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, United States; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, United States
推荐引用方式 GB/T 7714	D’Mello A.,Riegler A.N.,Martínez E.,等. An in vivo atlas of host–pathogen transcriptomes during Streptococcus pneumoniae colonization and disease[J],2021,117(52).
APA	D’Mello A..,Riegler A.N..,Martínez E..,Beno S.M..,Ricketts T.D..,...&Tettelin H..(2021).An in vivo atlas of host–pathogen transcriptomes during Streptococcus pneumoniae colonization and disease.Proceedings of the National Academy of Sciences of the United States of America,117(52).
MLA	D’Mello A.,et al."An in vivo atlas of host–pathogen transcriptomes during Streptococcus pneumoniae colonization and disease".Proceedings of the National Academy of Sciences of the United States of America 117.52(2021).