气候变化领域集成服务门户(CCPortal): Histone variants in archaea and the evolution of combinatorial chromatin complexity

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DOI	10.1073/PNAS.2007056117
	Histone variants in archaea and the evolution of combinatorial chromatin complexity
	Stevens K.M.; Swadling J.B.; Hocher A.; Bang C.; Gribaldo S.; Schmitz R.A.; Warnecke T.
发表日期	2021
ISSN	00278424
起始页码	33384
结束页码	33395
卷号	117 期号:52
英文摘要	Nucleosomes in eukaryotes act as platforms for the dynamic integration of epigenetic information. Posttranslational modifications are reversibly added or removed and core histones exchanged for paralogous variants, in concert with changing demands on transcription and genome accessibility. Histones are also common in archaea. Their role in genome regulation, however, and the capacity of individual paralogs to assemble into histone–DNA complexes with distinct properties remain poorly understood. Here, we combine structural modeling with phylogenetic analysis to shed light on archaeal histone paralogs, their evolutionary history, and capacity to generate combinatorial chromatin states through hetero-oligomeric assembly. Focusing on the human commensal Methanosphaera stadtmanae as a model archaeal system, we show that the heteromeric complexes that can be assembled from its seven histone paralogs vary substantially in DNA binding affinity and tetramer stability. Using molecular dynamics simulations, we go on to identify unique paralogs in M. stadtmanae and Methanobrevibacter smithii that are characterized by unstable interfaces between dimers. We propose that these paralogs act as capstones that prevent stable tetramer formation and extension into longer oligomers characteristic of model archaeal histones. Importantly, we provide evidence from phylogeny and genome architecture that these capstones, as well as other paralogs in the Methanobacteriales, have been maintained for hundreds of millions of years following ancient duplication events. Taken together, our findings indicate that at least some archaeal histone paralogs have evolved to play distinct and conserved functional roles, reminiscent of eukaryotic histone variants. We conclude that combinatorially complex histone-based chromatin is not restricted to eukaryotes and likely predates their emergence. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Archaea; Chromatin; Evolution; Histone variants
语种	英语
scopus关键词	amino acid; DNA; histone; protein binding; archaeon; chemistry; chromatin; genetic variation; genetics; metabolism; molecular dynamics; molecular evolution; mutation; phylogeny; Amino Acids; Archaea; Chromatin; DNA; Evolution, Molecular; Genetic Variation; Histones; Molecular Dynamics Simulation; Mutation; Phylogeny; Protein Binding
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179615
作者单位	Molecular Systems Group, Quantitative Biology Section, Medical Research Council London Institute of Medical Sciences, London, W12 0NN, United Kingdom; Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, W12 0NN, United Kingdom; Institute for General Microbiology, University of Kiel, Kiel, 24118, Germany; Institute of Clinical Molecular Biology, University of Kiel, Kiel, 24105, Germany; Department of Microbiology, Unit “Evolutionary Biology of the Microbial Cell,” Institut Pasteur, Paris, 75015, France
推荐引用方式 GB/T 7714	Stevens K.M.,Swadling J.B.,Hocher A.,et al. Histone variants in archaea and the evolution of combinatorial chromatin complexity[J],2021,117(52).
APA	Stevens K.M..,Swadling J.B..,Hocher A..,Bang C..,Gribaldo S..,...&Warnecke T..(2021).Histone variants in archaea and the evolution of combinatorial chromatin complexity.Proceedings of the National Academy of Sciences of the United States of America,117(52).
MLA	Stevens K.M.,et al."Histone variants in archaea and the evolution of combinatorial chromatin complexity".Proceedings of the National Academy of Sciences of the United States of America 117.52(2021).