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DOI	10.1073/PNAS.2019698117
	Upregulation of a KN1 homolog by transposon insertion promotes leafy head development in lettuce
	Yu C.; Yan C.; Liu Y.; Liu Y.; Jia Y.; Lavelle D.; An G.; Zhang W.; Zhang L.; Han R.; Larkin R.M.; Chen J.; Michelmore R.W.; Kuang H.
发表日期	2021
ISSN	00278424
起始页码	33668
结束页码	33678
卷号	117 期号:52
英文摘要	Leafy head is a unique type of plant architecture found in some vegetable crops, with leaves bending inward to form a compact head. The genetic and molecular mechanisms underlying leafy head in vegetables remain poorly understood. We genetically fine-mapped and cloned a major quantitative trait locus controlling heading in lettuce. The candidate gene (LsKN1) is a homolog of knotted 1 (KN1) from Zea mays. Complementation and CRISPR/Cas9 knockout experiments confirmed the role of LsKN1 in heading. In heading lettuce, there is a CACTA-like transposon inserted into the first exon of LsKN1 (LsKN1∇). The transposon sequences act as a promoter rather than an enhancer and drive high expression of LsKN1∇. The enhanced expression of LsKN1∇ is necessary but not sufficient for heading in lettuce. Data from ChIP-sequencing, electrophoretic mobility shift assays, and dual luciferase assays indicate that the LsKN1∇ protein binds the promoter of LsAS1 and down-regulates its expression to alter leaf dorsoventrality. This study provides insight into plant leaf development and will be useful for studies on heading in other vegetable crops. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Dorsoventrality; Leaf development; Leafy head; Transposon
语种	英语
scopus关键词	plant protein; protein binding; anatomy and histology; chemistry; gene duplication; gene expression regulation; genetic transcription; genetics; growth, development and aging; lettuce; nucleotide sequence; phylogeny; plant gene; plant leaf; promoter region; quantitative trait locus; sequence homology; transposon; upregulation; Base Sequence; DNA Transposable Elements; Gene Duplication; Gene Expression Regulation, Plant; Genes, Plant; Lettuce; Mutagenesis, Insertional; Phylogeny; Plant Leaves; Plant Proteins; Promoter Regions, Genetic; Protein Binding; Quantitative Trait Loci; Sequence Homology, Nucleic Acid; Transcription, Genetic; Up-Regulation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179610
作者单位	Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture (MOA), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China; Genome Center and Department of Plant Sciences, University of California, Davis, CA 95616, United States
推荐引用方式 GB/T 7714	Yu C.,Yan C.,Liu Y.,et al. Upregulation of a KN1 homolog by transposon insertion promotes leafy head development in lettuce[J],2021,117(52).
APA	Yu C..,Yan C..,Liu Y..,Liu Y..,Jia Y..,...&Kuang H..(2021).Upregulation of a KN1 homolog by transposon insertion promotes leafy head development in lettuce.Proceedings of the National Academy of Sciences of the United States of America,117(52).
MLA	Yu C.,et al."Upregulation of a KN1 homolog by transposon insertion promotes leafy head development in lettuce".Proceedings of the National Academy of Sciences of the United States of America 117.52(2021).