气候变化领域集成服务门户(CCPortal): Absorption of Naphthalene by Leaves and Its Interaction with Chlorophyll

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DOI	10.1134/S1021443720040093
	Absorption of Naphthalene by Leaves and Its Interaction with Chlorophyll–Protein Complexes of Pea Plants
	Khristin M.S.; Lankin A.V.; Kreslavski V.D.
发表日期	2020
ISSN	10214437
起始页码	646
结束页码	652
卷号	67 期号:4
英文摘要	Abstract: Mechanisms of photosynthesis inhibition by vaporous naphthalene, its permeation into thylakoids, and interactions with chlorophyll–protein complexes were studied. It was found that vaporous naphthalene is first adsorbed on the pea (Pisum sativum L.) leaf’s surface and then penetrates into the leaf, binds to thylakoids, and inhibits photosynthesis. The adsorbed naphthalene can be washed from the leaf surface by means of short-term (1 min) incubation of leaves in hexane. When thylakoids isolated from such leaves were exposed to UV light with the peak intensity at 266 nm, the emission bands at 325 nm and 335 nm corresponding to naphthalene fluorescence in water were detected. Nondenaturing PAGE was applied to isolate chlorophyll–protein complexes (CPC). In the gels thus obtained, neither of the green zones emitted naphthalene fluorescence, which was ascribed to fluorescence quenching by CPC components absorbing light at 266 nm. However, when CPC-containing gels were exposed to UV light, chlorophyll bleaching developed faster in naphthalene-treated than in naphthalene-free samples. In addition, excitation energy transfer from naphthalene to chlorophyll was detected in light-harvesting II (LHCII) trimer complexes after washing PAAG from low-molecular fluorescence quenchers. Thus, vaporous naphthalene accumulates on the leaf surface, partially penetrates into the leaves, and binds to thylakoids and CPC. It is assumed that naphthalene–LHCII trimer interactions facilitate energy transfer from naphthalene to chlorophyll, thereby enhancing chlorophyll photobleaching and inhibition of photosynthesis. © 2020, Pleiades Publishing, Ltd.
英文关键词	chlorophyll–protein complexes; energy transfer; fluorescence; leaves; naphthalene vapors; photosystem II; Pisum sativum; thylakoids; UV light
来源期刊	Russian Journal of Plant Physiology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176882
作者单位	Institute of Basic Problems of Biology, Russian Academy of Sciences, Pushchino, Moscow Oblast, Russian Federation; Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino, Moscow Oblast, Russian Federation; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation
推荐引用方式 GB/T 7714	Khristin M.S.,Lankin A.V.,Kreslavski V.D.. Absorption of Naphthalene by Leaves and Its Interaction with Chlorophyll–Protein Complexes of Pea Plants[J],2020,67(4).
APA	Khristin M.S.,Lankin A.V.,&Kreslavski V.D..(2020).Absorption of Naphthalene by Leaves and Its Interaction with Chlorophyll–Protein Complexes of Pea Plants.Russian Journal of Plant Physiology,67(4).
MLA	Khristin M.S.,et al."Absorption of Naphthalene by Leaves and Its Interaction with Chlorophyll–Protein Complexes of Pea Plants".Russian Journal of Plant Physiology 67.4(2020).