气候变化领域集成服务门户(CCPortal): Surface-enhanced electrochemiluminescence combined with resonance energy transfer for sensitive carcinoembryonic antigen detection in exhaled breath condensates

CCPortal

DOI	10.1039/d0an00864h
	Surface-enhanced electrochemiluminescence combined with resonance energy transfer for sensitive carcinoembryonic antigen detection in exhaled breath condensates
	Ding L.; Xu S.; Huang D.; Chen L.; Kannan P.; Guo L.; Lin Z.
发表日期	2020
ISSN	32654
起始页码	6524
结束页码	6531
卷号	145 期号:20
英文摘要	The detection of biomarkers in exhaled breath condensates (EBCs) is regarded as a promising non-invasive diagnostic approach. However, the ultralow concentration of biomarkers in EBCs is a great challenge. Herein, a sensitive dual signal amplification strategy was developed based on surface-enhanced electrochemiluminescence (SEECL) combined with resonance energy transfer (RET). Gold nanoparticles-functionalized graphite-like carbon nitride nanohybrids (Au-g-C3N4 NHs) could be used as an energy transfer donor because of the good overlap between its emission peak and the absorption peak of tris(2,2′-bipyridine)ruthenium dichloride (Ru(bpy)3Cl2) at 460 nm. Gold-silicon dioxide core-shell nanoparticles doped with Ru(bpy)32+(Au@SiO2-Ru) were employed as an energy transfer acceptor emitting at 620 nm. Moreover, the signals at 620 nm emitted by Ru (bpy)32+ were enhanced by 5 times, attributed to the localized surface plasmon resonance (LSPR) of gold nanoparticles (Au NPs). The detection of carcinoembryonic antigen (CEA) was performed by using two aptamers as the recognition unit; whereby aptamer 1 (Apt1) was modified on the surface of Au-g-C3N4 NHs, and aptamer 2 (Apt2) was banded on the surface of Au@SiO2-Ru. In the presence of CEA, a sandwich structure was formed between Au-g-C3N4 NHs-Apt1-CEA and Apt2-Au@SiO2-Ru, which resulted in an ultrasensitive detection of CEA. The proposed electrochemiluminescence sensor showed a wide linear relationship with the CEA concentration in the range from 1.0 pg mL-1 to 5.0 ng mL-1, with a limit of detection (LOD) of 0.3 pg mL-1. Finally, the practicality of the proposed sensor was demonstrated to detect CEA in EBCs, and the obtained results were in good agreement with the enzyme-linked immunosorbent assay (ELISA) method. This journal is © The Royal Society of Chemistry.
scopus关键词	Antigens; Biomarkers; Carbon nitride; Condensate treatment; Core shell nanoparticles; Energy transfer; Fiber optic sensors; Gold nanoparticles; Metal nanoparticles; Noninvasive medical procedures; Plasmons; Resonance; Ruthenium compounds; Silica; Silicon oxides; SiO2 nanoparticles; Surface plasmon resonance; Carcinoembryonic antigen; Electrochemiluminescence; Electrochemiluminescence sensors; Enzyme-linked immunosorbent assay methods; Exhaled breath condensate; Graphite-like carbon nitrides; Localized surface plasmon resonance; Resonance energy transfer; Gold compounds
来源期刊	Analyst
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176769
作者单位	Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou Fujian Province, 350116, China; College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang Province, 314001, China
推荐引用方式 GB/T 7714	Ding L.,Xu S.,Huang D.,et al. Surface-enhanced electrochemiluminescence combined with resonance energy transfer for sensitive carcinoembryonic antigen detection in exhaled breath condensates[J],2020,145(20).
APA	Ding L..,Xu S..,Huang D..,Chen L..,Kannan P..,...&Lin Z..(2020).Surface-enhanced electrochemiluminescence combined with resonance energy transfer for sensitive carcinoembryonic antigen detection in exhaled breath condensates.Analyst,145(20).
MLA	Ding L.,et al."Surface-enhanced electrochemiluminescence combined with resonance energy transfer for sensitive carcinoembryonic antigen detection in exhaled breath condensates".Analyst 145.20(2020).