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DOI	10.1007/s11090-020-10109-8
	Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma
	Tomeková J.; Kyzek S.; Medvecká V.; Gálová E.; Zahoranová A.
发表日期	2020
ISSN	2724324
起始页码	1571
结束页码	1584
卷号	40 期号:6
英文摘要	Cold atmospheric pressure plasma treatment is currently being explored as an alternative way to improve the germination and growing parameters of plant seeds. However, it is important to pay attention to the effect of plasma treatment on DNA damage of the seeds as well as detailed characteristics of plasma composition and parameters. The aim of this work was to study the DNA damage of plasma-treated pea seeds (Pisum sativum L.) and plasma parameters such as the chemical composition of plasma gaseous compounds and plasma radiation. Seeds were treated with plasma using the diffuse coplanar surface barrier discharge generated in different working gases (ambient air, nitrogen, oxygen and different mixtures of oxygen and nitrogen) at atmospheric pressure and at 60 s, 180 s and 300 s exposure times. DNA damage was studied using the single cell-gel electrophoresis called the comet assay and the plasma parameters were investigated by Fourier transform infrared spectroscopy and optical emission spectroscopy. Experiments in different ratios of oxygen and nitrogen were realized in order to understand the reaction mechanism between the ambient air plasma and the treated seeds. Based on our results, ambient air plasma appears to be the most advantageous for the plasma treatment due to no significant DNA damage because of the proper combination of plasma composition in combination with water vapor present in ambient air. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
英文关键词	DNA damage; Fourier transform infrared spectroscopy; Low-temperature plasma; Optical emission spectroscopy; Pea seeds
scopus关键词	Atmospheric chemistry; Atmospheric pressure; Cell culture; DNA; Electric discharges; Electrophoresis; Fourier transform infrared spectroscopy; Nitrogen; Optical emission spectroscopy; Oxygen; Plasma applications; Plasma devices; Seed; Atmospheric pressure plasma treatment; Atmospheric pressure plasmas; Chemical compositions; Optical diagnostics; Oxygen and nitrogens; Plasma composition; Single cell gel electrophoresis; Surface barrier discharge; Nitrogen plasma
来源期刊	Plasma Chemistry and Plasma Processing
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176764
作者单位	Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, Bratislava, 842 48, Slovakia; Department of Genetics, Faculty of Natural Sciences, Comenius University, Ilkovičova 6, Mlynská dolina, Bratislava, 842 15, Slovakia
推荐引用方式 GB/T 7714	Tomeková J.,Kyzek S.,Medvecká V.,et al. Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma[J],2020,40(6).
APA	Tomeková J.,Kyzek S.,Medvecká V.,Gálová E.,&Zahoranová A..(2020).Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma.Plasma Chemistry and Plasma Processing,40(6).
MLA	Tomeková J.,et al."Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma".Plasma Chemistry and Plasma Processing 40.6(2020).