气候变化领域集成服务门户(CCPortal): Comparison of the Postprandial Metabolic Fate of U-13C Stearic Acid and U-13C Oleic Acid in Postmenopausal Women

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DOI	10.1161/ATVBAHA.120.315260
	Comparison of the Postprandial Metabolic Fate of U-13C Stearic Acid and U-13C Oleic Acid in Postmenopausal Women
	Rodríguez-Morató J.; Galluccio J.; Dolnikowski G.G.; Lichtenstein A.H.; Matthan N.R.
发表日期	2020
ISSN	10795642
起始页码	2953
结束页码	2964
英文摘要	Objective: Compare the postprandial fatty acid metabolism of isotopically labeled stearate (U-13C18:0) and oleate (U-13C18:1). Approach and Results: In conjunction with a randomized-controlled crossover trial, 6 hypercholesterolemic postmenopausal women (≥50 years; body mass index: 25.6±3.0 kg/m2; LDL [low-density lipoprotein]-cholesterol ≥110 mg/dL) consumed isocaloric diets enriched in 18:0 or 18:1 (10%-15% E) for 5 weeks each. On day 1 of week 5, following a 12-hour fast, participants receive their experimental diet divided into 13 hourly meals beginning at 8 am. U-13C18:0 or U-13C18:1 was incorporated into the 1:00 pm meal (1.0 mg/kg body weight). Serial blood and breath samples were collected over 12 hours and fasting samples at 24 and 48 hours. Plasma and lipid subfraction fatty acid profiles were assessed by gas chromatography-flame ionization detector, isotope-enrichment by liquid chromatography time-of-flight mass spectrometry, and fatty acid oxidation rate (expired 13CO2) by isotope ratio mass spectrometry. Both diets resulted in similar plasma LDL-cholesterol concentrations. Kinetic curves showed that U-13C18:0 had a higher plasma area under the curve (66%), lower plasma clearance rate (-46%), and a lower cumulative oxidation rate (-34%) than U-13C18:1. Three labeled plasma metabolites of U-13C18:0 were detected: 13C16:0, 13C16:1, and 13C18:1. No plasma metabolites of U-13C18:1 were detected within the study time-frame. Higher incorporation of 18:0 in cholesteryl ester and triglyceride fractions was observed on the 18:0 compared with the 18:1 diet. Conclusions: The neutrality of 18:0 on plasma LDL-cholesterol concentrations is not attributable to a single factor. Compared with 18:1, 18:0 had higher plasma area under the curve because of lower clearance and oxidation rates, underwent both a direct and a multistage conversion to 18:1, and was preferentially incorporated into cholesteryl esters and triglycerides. © 2020 Lippincott Williams and Wilkins. All rights reserved.
英文关键词	diet; hypercholesterolemia; metabolism; oleic acid; post menopausal women; randomized controlled trial; stable-isotopes
scopus关键词	carbon; cholesterol ester; low density lipoprotein cholesterol; oleic acid; stearic acid; stearic acid derivative; triacylglycerol; aged; blood; controlled study; crossover procedure; female; gastrointestinal absorption; human; hypercholesterolemia; middle aged; oxidation reduction reaction; postmenopause; postprandial state; randomized controlled trial; very elderly; Aged; Aged, 80 and over; Carbon Isotopes; Cholesterol Esters; Cholesterol, LDL; Cross-Over Studies; Female; Gastrointestinal Absorption; Humans; Hypercholesterolemia; Middle Aged; Oleic Acid; Oxidation-Reduction; Postmenopause; Postprandial Period; Stearic Acids; Triglycerides
来源期刊	Arteriosclerosis, Thrombosis, and Vascular Biology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176718
作者单位	Cardiovascular and Mass Spectrometry Laboratories, Jean Mayer Usda Human Nutrition Research Center on Aging at Tufts University, Boston, MA, United States; Integrative Pharmacology and Systems Neuroscience Research Group, Imim (Hospital Del Mar Medical Research Institute), Barcelona, Spain
推荐引用方式 GB/T 7714	Rodríguez-Morató J.,Galluccio J.,Dolnikowski G.G.,et al. Comparison of the Postprandial Metabolic Fate of U-13C Stearic Acid and U-13C Oleic Acid in Postmenopausal Women[J],2020.
APA	Rodríguez-Morató J.,Galluccio J.,Dolnikowski G.G.,Lichtenstein A.H.,&Matthan N.R..(2020).Comparison of the Postprandial Metabolic Fate of U-13C Stearic Acid and U-13C Oleic Acid in Postmenopausal Women.Arteriosclerosis, Thrombosis, and Vascular Biology.
MLA	Rodríguez-Morató J.,et al."Comparison of the Postprandial Metabolic Fate of U-13C Stearic Acid and U-13C Oleic Acid in Postmenopausal Women".Arteriosclerosis, Thrombosis, and Vascular Biology (2020).