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DOI	10.1002/ps.5153
	(E)-β-farnesene synthase gene affects aphid behavior in transgenic Medicago sativa
	Wang X.; Gao Y.; Chen Z.; Li J.; Huang J.; Cao J.; Cui M.; Ban L.
发表日期	2019
ISSN	1526498X
起始页码	622
结束页码	631
卷号	75 期号:3
英文摘要	BACKGROUND: Pea aphid (Acyrthosiphon pisum Harris) is one of the major pests in alfalfa crops, causing significant yield losses. (E)-β-farnesene (EβF), an alarm pheromone released by pea aphid, is generic to many species of aphids, and is used to minimize potential danger from predators and parasitoids by avoiding the source of the pheromone. RESULTS: In this study, EβF synthase gene was constructed into a plant expression vector, and overexpressed in alfalfa (Medicago sativa L.), with expression among transgenic lines confirmed by qRT-PCR. Subcellular localization analysis showed that EβF synthase gene was expressed in the plasma membrane and nucleus of the leaf. GC/MS of extraction from transgenic alfalfa indicated emission of EβF ranging from 5.92 to 13.09 ng day −1 g −1 fresh tissue. Behavior assays in Y-olfactometers demonstrated that transgenic alfalfa expressing AaEβF gene could repel pea aphids, with aphids taking a significantly longer time to select a transgenic line compared with the control line (P < 0.01). CONCLUSION: We have demonstrated a potentially valuable strategy of using EβF synthase genes for aphid control in alfalfa. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry
英文关键词	(E)-β-farnesene synthase gene; aphid; behavior assay; gas chromatography mass spectrometry; Medicago sativa
scopus关键词	alfalfa; aphid; behavior; bioassay; crop pest; enzyme; enzyme activity; gas chromatography; gene; mass spectrometry; pheromone; transgenic plant; Acyrthosiphon pisum; Aphididae; Medicago sativa; beta-farnesene; beta-farnesene synthase; inorganic pyrophosphatase; pheromone; sesquiterpene; alfalfa; animal; animal behavior; aphid; genetics; metabolism; physiology; plant leaf; transgenic plant; Animals; Aphids; Behavior, Animal; Medicago sativa; Pheromones; Plant Leaves; Plants, Genetically Modified; Pyrophosphatases; Sesquiterpenes
来源期刊	Pest Management Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176483
作者单位	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China; College of Animal Science and Technology, China Agricultural University, Beijing, China; The Affiliated High School of Peking University, Beijing, China; National Animal Husbandry Service, Ministry of Agriculture, Beijing, China
推荐引用方式 GB/T 7714	Wang X.,Gao Y.,Chen Z.,et al. (E)-β-farnesene synthase gene affects aphid behavior in transgenic Medicago sativa[J],2019,75(3).
APA	Wang X..,Gao Y..,Chen Z..,Li J..,Huang J..,...&Ban L..(2019).(E)-β-farnesene synthase gene affects aphid behavior in transgenic Medicago sativa.Pest Management Science,75(3).
MLA	Wang X.,et al."(E)-β-farnesene synthase gene affects aphid behavior in transgenic Medicago sativa".Pest Management Science 75.3(2019).