气候变化领域集成服务门户(CCPortal): Electrochemical aptasensor based on conductive supramolecular polymer hydrogels for thrombin detection with high selectivity

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DOI	10.1016/j.talanta.2019.120140
	Electrochemical aptasensor based on conductive supramolecular polymer hydrogels for thrombin detection with high selectivity
	Wang X.; Gao F.; Gong Y.; Liu G.; Zhang Y.; Ding C.
发表日期	2019
ISSN	399140
卷号	205
英文摘要	Herein, we synthesized a kind of conductive supramolecular polymer hydrogel (CSPH) based on polyaniline (PANI) which can not only improve the conductivity but also promote antifouling performance of the aptasensor for the specific recruitment of thrombin (TB) from complex samples. With the electrochemical copolymerization of aniline (AN) and 3-aminophenylboronic acid (ABA) on glassy carbon electrode (GCE), the electrode was then inserted into the polyvinyl alcohol (PVA) solution to obtain robust CSPH through boric acid groups incorporated onto PANI to cause gelation of PVA solution, owing to the hydrophilicity of CSPH and nearly electrical neutrality, the modified electrode is antifouling without integration of other antifouling materials. A sandwich-type electrochemical aptasensor was constructed on the CSPH based electrode interfaces. Thrombin aptamer 1 (TBA1) were modified on the CSPH through amide bond, and thrombin aptamer 2 modified magnetic nanoparticles (MNP-TBA2) are used as signal amplification probes, the aptasensor has good sensitivity with a linear range from 1 pmol/L to 10 nmol/L and has a detection limit down to 0.64 pmol/L. The strategy of utilizing eletropolymerization of CSPH films to undergo highly selective thrombin recognition is, of course, readily extended to a broad range of targets in the real samples, and the recovery was ranging from 95.2% to 106.3% and RSDs varying from 2.3% to 4.5%. Elsevier B.V.
英文关键词	Antifouling; Conducting supramolecular polymer hydrogel (CSPH); Electrochemical aptasensor; Polyaniline; Thrombin
scopus关键词	Amides; Aniline; Boric acid; Gelation; Glass membrane electrodes; Hydrogels; Hydrophilicity; Nanomagnetics; Nanoparticles; Polyaniline; Supramolecular chemistry; 3-Aminophenylboronic acid; Antifouling; Electrochemical aptasensor; Electrochemical copolymerization; Glassy carbon electrodes; Poly (vinyl alcohol) (PVA); Supramolecular polymers; Thrombin; Enzymes; aniline derivative; aptamer; polyaniline; thrombin; thrombin aptamer; biofouling; chemistry; electric conductivity; electrochemistry; genetic procedures; human; hydrogel; limit of detection; metabolism; prevention and control; procedures; Aniline Compounds; Aptamers, Nucleotide; Biofouling; Biosensing Techniques; Electric Conductivity; Electrochemistry; Humans; Hydrogels; Limit of Detection; Thrombin
来源期刊	Talanta
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176153
作者单位	Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China
推荐引用方式 GB/T 7714	Wang X.,Gao F.,Gong Y.,et al. Electrochemical aptasensor based on conductive supramolecular polymer hydrogels for thrombin detection with high selectivity[J],2019,205.
APA	Wang X.,Gao F.,Gong Y.,Liu G.,Zhang Y.,&Ding C..(2019).Electrochemical aptasensor based on conductive supramolecular polymer hydrogels for thrombin detection with high selectivity.Talanta,205.
MLA	Wang X.,et al."Electrochemical aptasensor based on conductive supramolecular polymer hydrogels for thrombin detection with high selectivity".Talanta 205(2019).