气候变化领域集成服务门户(CCPortal): In-situ rapid bioaerosol detection in the ambient air by miniature multiplex PCR utilizing technique

CCPortal

DOI	10.1016/j.atmosenv.2020.118147
	In-situ rapid bioaerosol detection in the ambient air by miniature multiplex PCR utilizing technique
	Agranovski I.E.; Usachev E.V.
发表日期	2021
ISSN	1352-2310
卷号	246
英文摘要	Given the current problems associated with the COVID-19 pandemic, research in the field of rapid and reliable detection and characterization of airborne pathogenic microorganisms is becoming of exceptional urgency. This project is focused on development of portable first alert bioaerosol monitoring technique utilizing personal bioaerosol sampler capable of high efficient trapping of airborne microorganisms into collecting liquid along with a laser based mini Polymerase Chain Reaction (PCR) machine in a multiplex format (multi-target simultaneous detection) with following identification of minimal time period required for detection of any airborne microbial targets in the ambient air. The experimental program was undertaken under controlled laboratory conditions utilizing of up to 7 plex PCR format for detection of airborne microbial targets in the dynamic aerosol chamber. The technology demonstrated efficient operation and was capable of detecting airborne microbial targets during even shortest experimental sampling periods of 10 s. The total time of detection/analysis was less than 40 min. The proposed sampling timeframe represents realistic scenario of bioaerosol exposure of humans’ moving through areas potentially contaminated by bioaerosol originated from natural or anthropogenic sources. The research outcomes look very promising enabling development of new generation of portable, reliable and fast bioaerosol monitors, which are unique and highly demanded in the areas of defence, public health and others. © 2020 Elsevier Ltd
英文关键词	Aerosols; Microorganisms; Airborne microorganisms; Anthropogenic sources; Bio-aerosol detections; Bioaerosol monitoring; Controlled laboratories; Experimental program; Pathogenic microorganisms; Simultaneous detection; Polymerase chain reaction; fluorescent dye; virus RNA; aerosol; ambient air; detection method; in situ measurement; pollution monitoring; polymerase chain reaction; aerosol; airborne microorganism; ambient air; Article; controlled study; duplex polymerase chain reaction; Enterobacteria phage T4; Escherichia coli; fluorescence; Human adenovirus 5; Influenza A virus (H1N1); light; multiplex polymerase chain reaction; nonhuman; priority journal; real time polymerase chain reaction; virus genome; Varanidae
语种	英语
来源期刊	Atmospheric Environment
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/169068
作者单位	School of Engineering and Built Environment, Griffith University, Nathan, QLD 4111, Australia; N.F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Moscow, 123098, Russian Federation
推荐引用方式 GB/T 7714	Agranovski I.E.,Usachev E.V.. In-situ rapid bioaerosol detection in the ambient air by miniature multiplex PCR utilizing technique[J],2021,246.
APA	Agranovski I.E.,&Usachev E.V..(2021).In-situ rapid bioaerosol detection in the ambient air by miniature multiplex PCR utilizing technique.Atmospheric Environment,246.
MLA	Agranovski I.E.,et al."In-situ rapid bioaerosol detection in the ambient air by miniature multiplex PCR utilizing technique".Atmospheric Environment 246(2021).