气候变化领域集成服务门户(CCPortal): Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria

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DOI	10.1039/c9ee01214a
	Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria
	Liu X.; Miao R.; Lindberg P.; Lindblad P.
发表日期	2019
ISSN	1754-5692
起始页码	2765
结束页码	2777
卷号	12 期号:9
英文摘要	Cyanobacteria are photoautotrophic microorganisms which can be engineered to directly convert CO2 and water into biofuels and chemicals via photosynthesis using sunlight as energy. However, the product titers and rates are the main challenges that need to be overcome for industrial applications. Here we present systematic modular engineering of the cyanobacterium Synechocystis PCC 6803, enabling efficient biosynthesis of 1-butanol, an attractive commodity chemical and gasoline substitute. Through introducing and re-casting the 1-butanol biosynthetic pathway at the gene and enzyme levels, optimizing the 5′-regions of expression units for tuning transcription and translation, rewiring the carbon flux and rewriting the photosynthetic central carbon metabolism to enhance the precursor supply, and performing process development, we were able to reach a cumulative 1-butanol titer of 4.8 g L-1 with a maximal rate of 302 mg L-1 day-1 from the engineered Synechocystis. This represents the highest 1-butanol production from CO2 reported so far. Our multi-level modular strategy for high-level production of chemicals and advanced biofuels represents a blue-print for future systematic engineering in photosynthetic microorganisms. © 2019 The Royal Society of Chemistry.
语种	英语
scopus关键词	Biochemistry; Biofuels; Carbon dioxide; Microorganisms; Transcription; Biosynthetic pathway; Central carbon metabolisms; Commodity chemicals; Cyanobacterium synechocystis; High-level production; Photosynthetic microorganisms; Process development; Systematic engineering; Biosynthesis; bioengineering; biofuel; carbon flux; chemical compound; commodity; cyanobacterium; enzyme; enzyme activity; metabolism; microorganism; photosynthesis; solar radiation; Cyanobacteria; Synechocystis; Synechocystis sp.; Synechocystis sp. PCC 6803
来源期刊	Energy and Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/162651
作者单位	Microbial Chemistry, Department of Chemistry-Ångström, Uppsala University, Box 523, Uppsala, SE-751 20, Sweden
推荐引用方式 GB/T 7714	Liu X.,Miao R.,Lindberg P.,et al. Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria[J],2019,12(9).
APA	Liu X.,Miao R.,Lindberg P.,&Lindblad P..(2019).Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria.Energy and Environmental Science,12(9).
MLA	Liu X.,et al."Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria".Energy and Environmental Science 12.9(2019).