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DOI10.1146/annurev-marine-010419-010714
NanoSIMS: Microscale Quantification of Biogeochemical Activity with Large-Scale Impacts
Mayali X.
发表日期2020
ISSN1941-1405
起始页码449
结束页码467
卷号12
英文摘要One major objective of aquatic microbial ecology is to understand the distribution of microbial populations over space and time and in response to environmental factors. Perhaps more importantly, it is crucial to quantify how those microbial cells affect biogeochemical processes of interest, such as primary production, nitrogen cycling, or the breakdown of pollutants. One valuable approach to link microbial identity to activity is to carry out incubations with stable-isotope-labeled substrates and then quantify the isotope incorporation by individual microbial cells using nanoscale secondary ion mass spectrometry (NanoSIMS). This review summarizes recent efforts in this field, highlights novel methods, describes studies investigating rare metabolisms as well as widespread microbial activity, and hopes to provide a framework to increase the use and capabilities of NanoSIMS for microbial biogeochemical studies in the future. © 2020 by Annual Reviews. All rights reserved.
英文关键词mass spectrometry; microbial ecology; NanoSIMS; stable-isotope probing
语种英语
scopus关键词Ecology; Isotopes; Mass spectrometry; Microbiology; Secondary ion mass spectrometry; Biogeochemical process; Biogeochemical studies; Environmental factors; Microbial activities; Microbial ecology; Microbial populations; NanoSIMS; Stable-isotope probing; Biogeochemistry; biogeochemistry; mass spectrometry; microbial activity; microbial ecology; nanocomposite; stable isotope; sea water; aquatic species; chemistry; devices; drug effect; environmental monitoring; growth, development and aging; isotope labeling; metabolism; microbiology; microflora; procedures; secondary ion mass spectrometry; toxicity; water pollutant; Aquatic Organisms; Environmental Monitoring; Isotope Labeling; Microbiota; Seawater; Spectrometry, Mass, Secondary Ion; Water Microbiology; Water Pollutants, Chemical
来源期刊Annual Review of Marine Science
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/161960
作者单位Lawrence Livermore National Laboratory, Livermore, CA 94550, United States
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Mayali X.. NanoSIMS: Microscale Quantification of Biogeochemical Activity with Large-Scale Impacts[J],2020,12.
APA Mayali X..(2020).NanoSIMS: Microscale Quantification of Biogeochemical Activity with Large-Scale Impacts.Annual Review of Marine Science,12.
MLA Mayali X.."NanoSIMS: Microscale Quantification of Biogeochemical Activity with Large-Scale Impacts".Annual Review of Marine Science 12(2020).
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