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DOI	10.1073/pnas.1918232117
	Footprints of natural selection at the mannose-6phosphate isomerase locus in barnacles
	Nunez J.C.B.; Flight P.A.; Neil K.B.; Rong S.; Eriksson L.A.; Ferranti D.A.; Rosenblad M.A.; Blomberg A.; Rand D.M.
发表日期	2020
ISSN	0027-8424
起始页码	5376
结束页码	5385
卷号	117 期号:10
英文摘要	The mannose-6-phosphate isomerase (Mpi) locus in Semibalanus balanoides has been studied as a candidate gene for balancing selection for more than two decades. Previous work has shown that Mpi allozyme genotypes (fast and slow) have different frequencies across Atlantic intertidal zones due to selection on post-settlement survival (i.e., allele zonation). We present the complete gene sequence of the Mpi locus and quantify nucleotide polymorphism in S. balanoides, as well as divergence to its sister taxon Semibalanus cariosus. We show that the slow allozyme contains a derived charge-altering amino acid polymorphism, and both allozyme classes correspond to two haplogroups with multiple internal haplotypes. The locus shows several footprints of balancing selection around the fast/slow site: an enrichment of positive Tajima’s D for nonsynonymous mutations, an excess of polymorphism, and a spike in the levels of silent polymorphism relative to silent divergence, as well as a site frequency spectrum enriched for midfrequency mutations. We observe other departures from neutrality across the locus in both coding and noncoding regions. These include a nonsynonymous trans-species polymorphism and a recent mutation under selection within the fast haplogroup. The latter suggests ongoing allelic replacement of functionally relevant amino acid variants. Moreover, predicted models of Mpi protein structure provide insight into the functional significance of the putatively selected amino acid polymorphisms. While footprints of selection are widespread across the range of S. balanoides, our data show that intertidal zonation patterns are variable across both spatial and temporal scales. These data provide further evidence for heterogeneous selection on Mpi. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Balancing selection; Barnacles; Ecological genomics; Population genetics; Semibalanus balanoides
语种	英语
scopus关键词	alloenzyme; amino acid; mannose phosphate isomerase; isoenzyme; mannose phosphate isomerase; 5' untranslated region; allele; amino acid polymorphism; Article; controlled study; DNA footprinting; exon; gene frequency; gene function; gene locus; gene mutation; gene sequence; gene structure; genetic code; genetic heterogeneity; genetic variability; genotype; haplotype; Mpi gene; nonhuman; priority journal; protein structure; Semibalanus balanoides; Semibalanus cariosus; single nucleotide polymorphism; taxon; animal; barnacle; chemistry; enzymology; gene locus; genetic polymorphism; genetic selection; genetics; mutation; Alleles; Animals; Genetic Loci; Genotype; Isoenzymes; Mannose-6-Phosphate Isomerase; Mutation; Polymorphism, Genetic; Selection, Genetic; Thoracica
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/161030
作者单位	Nunez, J.C.B., Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, United States; Flight, P.A., Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, United States; Neil, K.B., Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, United States; Rong, S., Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, United States, Center for Computational Molecular Biology, Brown University, Providence, RI 02912, United States; Eriksson, L.A., Department of Chemistry and Molecular Biology, Lundberg Laboratory, University of Gothenburg, Göteborg, 405 30, Sweden; Ferranti, D.A., Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, United States; Rosenblad, M.A., Department of Chemistry and Molecular Biology, Lundberg Laboratory, University of Gothenburg, Göteborg, 405 30, Sweden; Blomberg, A., Department of Chemistry and Molecular Biology,...
推荐引用方式 GB/T 7714	Nunez J.C.B.,Flight P.A.,Neil K.B.,et al. Footprints of natural selection at the mannose-6phosphate isomerase locus in barnacles[J],2020,117(10).
APA	Nunez J.C.B..,Flight P.A..,Neil K.B..,Rong S..,Eriksson L.A..,...&Rand D.M..(2020).Footprints of natural selection at the mannose-6phosphate isomerase locus in barnacles.Proceedings of the National Academy of Sciences of the United States of America,117(10).
MLA	Nunez J.C.B.,et al."Footprints of natural selection at the mannose-6phosphate isomerase locus in barnacles".Proceedings of the National Academy of Sciences of the United States of America 117.10(2020).