气候变化领域集成服务门户(CCPortal): DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness

CCPortal

DOI	10.1073/pnas.1918741117
	DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness
	Bush A.; Monk W.A.; Compson Z.G.; Peters D.L.; Porter T.M.; Shokralla S.; Wright M.T.G.; Hajibabaei M.; Baird D.J.
发表日期	2020
ISSN	0027-8424
起始页码	8539
结束页码	8545
卷号	117 期号:15
英文摘要	The complexity and natural variability of ecosystems present a challenge for reliable detection of change due to anthropogenic influences. This issue is exacerbated by necessary trade-offs that reduce the quality and resolution of survey data for assessments at large scales. The Peace-Athabasca Delta (PAD) is a large inland wetland complex in northern Alberta, Canada. Despite its geographic isolation, the PAD is threatened by encroachment of oil sands mining in the Athabasca watershed and hydroelectric dams in the Peace watershed. Methods capable of reliably detecting changes in ecosystem health are needed to evaluate and manage risks. Between 2011 and 2016, aquatic macroinvertebrates were sampled across a gradient of wetland flood frequency, applying both microscope-based morphological identification and DNA metabarcoding. By using multispecies occupancy models, we demonstrate that DNA metabarcoding detected a much broader range of taxa and more taxa per sample compared to traditional morphological identification and was essential to identifying significant responses to flood and thermal regimes. We show that family-level occupancy masks high variation among genera and quantify the bias of barcoding primers on the probability of detection in a natural community. Interestingly, patterns of community assembly were nearly random, suggesting a strong role of stochasticity in the dynamics of the metacommunity. This variability seriously compromises effective monitoring at local scales but also reflects resilience to hydrological and thermal variability. Nevertheless, simulations showed the greater efficiency of metabarcoding, particularly at a finer taxonomic resolution, provided the statistical power needed to detect change at the landscape scale. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Detectability; Occupancy; Power analysis; Stochasticity; Taxonomic resolution
语种	英语
scopus关键词	aquatic species; Article; biodiversity; biological monitoring; Canada; community structure; controlled study; DNA barcoding; endangered species; environmental change; environmental monitoring; nonhuman; null hypothesis; priority journal; risk management; watershed; wetland; wilderness; animal; biodiversity; DNA barcoding; ecosystem; invertebrate; physiology; procedures; wilderness; DNA; Animals; Biodiversity; DNA; DNA Barcoding, Taxonomic; Ecosystem; Environmental Monitoring; Invertebrates; Wetlands; Wilderness
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160991
作者单位	Bush, A., Environment and Climate Change Canada, Canadian Rivers Institute, Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada, Lancaster Environment Centre, University of Lancaster, Lancaster, LA1 4YW, United Kingdom; Monk, W.A., Environment and Climate Change Canada, Canadian Rivers Institute, Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, NB E3B 5A3, Canada; Compson, Z.G., Environment and Climate Change Canada, Canadian Rivers Institute, Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada, Centre for Environmental Genomics Applications, St. John's, NL A1A 0R6, Canada; Peters, D.L., Watershed Hydrology and Ecology Research Division, Environment and Climate Change Canada, University of Victoria, Victoria, BC V8P 5C2, Canada; Porter, T.M., Great Lakes Forestry Centre, Natural Resources Canada, Sault Ste. Marie, ON P6A 2E5, Canada, Centre for Biodiversity Genomics, University of Guelph, Guelp...
推荐引用方式 GB/T 7714	Bush A.,Monk W.A.,Compson Z.G.,et al. DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness[J],2020,117(15).
APA	Bush A..,Monk W.A..,Compson Z.G..,Peters D.L..,Porter T.M..,...&Baird D.J..(2020).DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness.Proceedings of the National Academy of Sciences of the United States of America,117(15).
MLA	Bush A.,et al."DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness".Proceedings of the National Academy of Sciences of the United States of America 117.15(2020).