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DOI	10.1073/pnas.2011299117
	Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs
	Versantvoort W.; Pol A.; Jetten M.S.M.; van Niftrik L.; Reimann J.; Kartal B.; Op den Camp H.J.M.
发表日期	2020
ISSN	0027-8424
起始页码	24459
结束页码	24463
卷号	117 期号:39
英文摘要	Aerobic and nitrite-dependent methanotrophs make a living from oxidizing methane via methanol to carbon dioxide. In addition, these microorganisms cometabolize ammonia due to its structural similarities to methane. The first step in both of these processes is catalyzed by methane monooxygenase, which converts methane or ammonia into methanol or hydroxylamine, respectively. Methanotrophs use methanol for energy conservation, whereas toxic hydroxylamine is a potent inhibitor that needs to be rapidly removed. It is suggested that many methanotrophs encode a hydroxylamine oxidoreductase (mHAO) in their genome to remove hydroxylamine, although biochemical evidence for this is lacking. HAOs also play a crucial role in the metabolism of aerobic and anaerobic ammonia oxidizers by converting hydroxylamine to nitric oxide (NO). Here, we purified an HAO from the thermophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV and characterized its kinetic properties. This mHAO possesses the characteristic P460 chromophore and is active up to at least 80 °C. It catalyzes the rapid oxidation of hydroxylamine to NO. In methanotrophs, mHAO efficiently removes hydroxylamine, which severely inhibits calcium-dependent, and as we show here, lanthanide-dependent methanol dehydrogenases, which are more prevalent in the environment. Our results indicate that mHAO allows methanotrophs to thrive under high ammonia concentrations in natural and engineered ecosystems, such as those observed in rice paddy fields, landfills, or volcanic mud pots, by preventing the accumulation of inhibitory hydroxylamine. Under oxic conditions, methanotrophs mainly oxidize ammonia to nitrite, whereas in hypoxic and anoxic environments reduction of both ammonia-derived nitrite and NO could lead to nitrous oxide (N2O) production. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Ammonia oxidation; Hydroxylamine oxidoreductase; Methanotrophy; Multiheme cytochrome; Nitric oxide
语种	英语
scopus关键词	ammonia; hydroxylamine; hydroxylamine oxidase; lanthanide; methane; methanol dehydrogenase; nitric oxide; aerobic ammonia oxidizer; aerobic metabolism; anaerobic ammonium oxidation; Article; catalysis; chromatophore; concentration (parameter); ecosystem; environment; kinetics; landfill; methanotrophic bacterium; Methylacidiphilum fumariolicum; nonhuman; priority journal; purification; rice; thermophilic bacterium; volcano
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160790
作者单位	Versantvoort, W., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Pol, A., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Jetten, M.S.M., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; van Niftrik, L., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Reimann, J., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Kartal, B., Microbial Physiology Group, Max Planck Institute for Marine Microbiology, Bremen, 28359, Germany; Op den Camp, H.J.M., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud Universi...
推荐引用方式 GB/T 7714	Versantvoort W.,Pol A.,Jetten M.S.M.,et al. Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs[J],2020,117(39).
APA	Versantvoort W..,Pol A..,Jetten M.S.M..,van Niftrik L..,Reimann J..,...&Op den Camp H.J.M..(2020).Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs.Proceedings of the National Academy of Sciences of the United States of America,117(39).
MLA	Versantvoort W.,et al."Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs".Proceedings of the National Academy of Sciences of the United States of America 117.39(2020).