气候变化领域集成服务门户(CCPortal): Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor

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DOI	10.1073/pnas.1920779117
	Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor
	Cleves P.A.; Tinoco A.I.; Bradford J.; Perrin D.; Bay L.K.; Pringle J.R.
发表日期	2020
ISSN	0027-8424
起始页码	28899
结束页码	28905
卷号	117 期号:46
英文摘要	Reef-building corals are keystone species that are threatened by anthropogenic stresses including climate change. To investigate corals’ responses to stress and other aspects of their biology, numerous genomic and transcriptomic studies have been performed, generating many hypotheses about the roles of particular genes and molecular pathways. However, it has not generally been possible to test these hypotheses rigorously because of the lack of genetic tools for corals or closely related cnidarians. CRISPR technology seems likely to alleviate this problem. Indeed, we show here that microinjection of single-guide RNA/Cas9 ribonucleoprotein complexes into fertilized eggs of the coral Acropora millepora can produce a sufficiently high frequency of mutations to detect a clear phenotype in the injected generation. Based in part on experiments in a sea-anemone model system, we targeted the gene encoding Heat Shock Transcription Factor 1 (HSF1) and obtained larvae in which >90% of the gene copies were mutant. The mutant larvae survived well at 27 °C but died rapidly at 34 °C, a temperature that did not produce detectable mortality over the duration of the experiment in wild-type (WT) larvae or larvae injected with Cas9 alone. We conclude that HSF1 function (presumably its induction of genes in response to heat stress) plays an important protective role in corals. More broadly, we conclude that CRISPR mutagenesis in corals should allow wide-ranging and rigorous tests of gene function in both larval and adult corals. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Acropora \| genome editing \| heat stress
语种	英语
scopus关键词	CRISPR associated endonuclease Cas9; guide RNA; heat shock transcription factor 1; ribonucleoprotein; Acropora millepora; animal experiment; animal tissue; Article; clustered regularly interspaced short palindromic repeat; controlled study; CRISPR-CAS9 system; embryo; gene dosage; gene editing; gene function; gene induction; gene mutation; heat stress; heat tolerance; HSF1 gene; larva; microinjection; mortality; mutagenesis; mutation rate; nonhuman; phenotype; priority journal; sea anemone; survival; zygote
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160724
作者单位	Cleves, P.A., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Tinoco, A.I., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Bradford, J., Science and Engineering Faculty, Queensland University of Technology, Brisbane, QLD 4001, Australia; Perrin, D., Science and Engineering Faculty, Queensland University of Technology, Brisbane, QLD 4001, Australia; Bay, L.K., Australian Institute of Marine Science, Townsville, QLD 4810, Australia; Pringle, J.R., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States
推荐引用方式 GB/T 7714	Cleves P.A.,Tinoco A.I.,Bradford J.,et al. Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor[J],2020,117(46).
APA	Cleves P.A.,Tinoco A.I.,Bradford J.,Perrin D.,Bay L.K.,&Pringle J.R..(2020).Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor.Proceedings of the National Academy of Sciences of the United States of America,117(46).
MLA	Cleves P.A.,et al."Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor".Proceedings of the National Academy of Sciences of the United States of America 117.46(2020).