气候变化领域集成服务门户(CCPortal): Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes

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DOI	10.1073/pnas.1612322114
	Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes
	Andreu-Fernández V.; Sancho M.; Genovés A.; Lucendo E.; Todt F.; Lauterwasser J.; Funk K.; Jahreis G.; Pérez-Payá E.; Mingarro I.; Edlich F.; Orzáez M.
发表日期	2017
ISSN	0027-8424
起始页码	310
结束页码	315
卷号	114 期号:2
英文摘要	The Bcl-2 (B-cell lymphoma 2) protein Bax (Bcl-2 associated X, apoptosis regulator) can commit cells to apoptosis via outer mitochondrial membrane permeabilization. Bax activity is controlled in healthy cells by prosurvival Bcl-2 proteins. C-terminal Bax transmembrane domain interactions were implicated recently in Bax pore formation. Here, we show that the isolated transmembrane domains of Bax, Bcl-xL (B-cell lymphoma-extra large), and Bcl-2 can mediate interactions between Bax and prosurvival proteins inside the membrane in the absence of apoptotic stimuli. Bcl-2 protein transmembrane domains specifically homooligomerize and heterooligomerize in bacterial and mitochondrial membranes. Their interactions participate in the regulation of Bcl-2 proteins, thus modulating apoptotic activity. Our results suggest that interactions between the transmembrane domains of Bax and antiapoptotic Bcl-2 proteins represent a previously unappreciated level of apoptosis regulation.
英文关键词	Apoptosis; Bcl-2; Mitochondria; Oligomerization; Transmembrane
语种	英语
scopus关键词	protein Bax; protein bcl 2; protein bcl xl; apoptosis regulatory protein; membrane protein; protein Bax; protein bcl x; protein binding; apoptosis; bacterial membrane; carboxy terminal sequence; Conference Paper; controlled study; human; human cell; membrane permeability; mitochondrial membrane; nonhuman; oligomerization; outer membrane; priority journal; protein domain; protein protein interaction; cell membrane; Escherichia coli; HCT 116 cell line; metabolism; mitochondrion; physiology; protein domain; tumor cell line; Apoptosis; Apoptosis Regulatory Proteins; bcl-2-Associated X Protein; bcl-X Protein; Cell Line, Tumor; Cell Membrane; Escherichia coli; HCT116 Cells; Humans; Membrane Proteins; Mitochondria; Mitochondrial Membranes; Protein Binding; Protein Domains
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160672
作者单位	Andreu-Fernández, V., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain, Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany, Departament de Bioquímica i Biologia Molecular, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina, Universitat de València, Burjassot, 46100, Spain; Sancho, M., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain; Genovés, A., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain; Lucendo, E., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain; Todt, F., Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany; Lauterwasser, J., Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany, Faculty of Biol...
推荐引用方式 GB/T 7714	Andreu-Fernández V.,Sancho M.,Genovés A.,et al. Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes[J],2017,114(2).
APA	Andreu-Fernández V..,Sancho M..,Genovés A..,Lucendo E..,Todt F..,...&Orzáez M..(2017).Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes.Proceedings of the National Academy of Sciences of the United States of America,114(2).
MLA	Andreu-Fernández V.,et al."Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes".Proceedings of the National Academy of Sciences of the United States of America 114.2(2017).