气候变化领域集成服务门户(CCPortal): Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth

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DOI	10.1073/pnas.1614380114
	Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth
	Weller B.; Zourelidou M.; Frank L.; Barbosa I.C.R.; Fastner A.; Richter S.; Jürgens G.; Hammes U.Z.; Schwechheimer C.
发表日期	2017
ISSN	0027-8424
起始页码	E887
结束页码	E896
卷号	114 期号:5
英文摘要	The directional distribution of the phytohormone auxin is essential for plant development. Directional auxin transport is mediated by the polarly distributed PIN-FORMED (PIN) auxin efflux carriers. We have previously shown that efficient PIN1-mediated auxin efflux requires activation through phosphorylation at the four serines S1-S4 in Arabidopsis thaliana. The Brefeldin A (BFA)-sensitive D6 PROTEIN KINASE (D6PK) and the BFA-insensitive PINOID (PID) phosphorylate and activate PIN1 through phosphorylation at all four phosphosites. PID, but not D6PK, can also induce PIN1 polarity shifts, seemingly through phosphorylation at S1-S3. The differential effects of D6PK and PID on PIN1 polarity had so far been attributed to their differential phosphosite preference for the four PIN1 phosphosites. We have mapped PIN1 phosphorylation at S1-S4 in situ using phosphosite-specific antibodies. We detected phosphorylation at PIN1 phosphosites at the basal (rootward) as well as the apical (shootward) plasma membrane in different root cell types, in embryos, and shoot apical meristems. Thereby, PIN1 phosphorylation at all phosphosites generally followed the predominant PIN1 distribution but was not restricted to specific polar sides of the cells. PIN1 phosphorylation at the basal and apical plasma membrane was differentially sensitive to BFA treatments, suggesting the involvement of different protein kinases or trafficking mechanisms in PIN1 phosphorylation control. We conclude that phosphosite preferences are not sufficient to explain the differential effects of D6PK and PID on PIN1 polarity, and suggest that a more complex model is needed to explain the effects of PID.
英文关键词	Arabidopsis; Auxin transport; PIN1; Polarity; Protein kinase
语种	英语
scopus关键词	auxin; brefeldin A; cantharidin; cell antibody; phosphatase; protein kinase; protein kinase D; protein kinase D6; protein kinase PINOID; serine; unclassified drug; Arabidopsis protein; brefeldin A; carrier protein; D6PK protein, Arabidopsis; indoleacetic acid derivative; PIN1 protein, Arabidopsis; PINOID protein, Arabidopsis; protein serine threonine kinase; antibiotic sensitivity; apical meristem; Arabidopsis thaliana; auxin transport; cell membrane; cell migration; cell polarity; cellular distribution; Conference Paper; embryo (anatomy); enzyme activation; gene mapping; gene mutation; gravitropism; molecular dynamics; nonhuman; plant epidermis cell; plant growth; plant root; priority journal; protein dephosphorylation; protein domain; protein expression; protein localization; protein phosphorylation; root cell; shoot; amino acid sequence; antagonists and inhibitors; antibody specificity; Arabidopsis; cell membrane; drug effect; growth, development and aging; meristem; metabolism; phosphorylation; plant structures; protein processing; protein transport; sequence alignment; transport at the cellular level; Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Biological Transport; Brefeldin A; Cell Membrane; Cell Polarity; Indoleacetic Acids; Membrane Transport Proteins; Meristem; Organ Specificity; Phosphorylation; Plant Structures; Protein Kinases; Protein Processing, Post-Translational; Protein Transport; Protein-Serine-Threonine Kinases; Sequence Alignment
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160665
作者单位	Weller, B., Plant Systems Biology, Technical University of Munich, Freising, 85354, Germany; Zourelidou, M., Plant Systems Biology, Technical University of Munich, Freising, 85354, Germany; Frank, L., Plant Systems Biology, Technical University of Munich, Freising, 85354, Germany, Research Unit Environmental Simulation, Helmholtz Zentrum München, Neuherberg, 85764, Germany; Barbosa, I.C.R., Plant Systems Biology, Technical University of Munich, Freising, 85354, Germany; Fastner, A., Department of Cell Biology and Biochemistry, Regensburg University, Regensburg, 93053, Germany; Richter, S., Developmental Genetics, Center for Plant Molecular Biology, Tübingen, 72076, Germany; Jürgens, G., Developmental Genetics, Center for Plant Molecular Biology, Tübingen, 72076, Germany; Hammes, U.Z., Department of Cell Biology and Biochemistry, Regensburg University, Regensburg, 93053, Germany; Schwechheimer, C., Plant Systems Biology, Technical University of Munich, Freising, 85354, Germany
推荐引用方式 GB/T 7714	Weller B.,Zourelidou M.,Frank L.,et al. Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth[J],2017,114(5).
APA	Weller B..,Zourelidou M..,Frank L..,Barbosa I.C.R..,Fastner A..,...&Schwechheimer C..(2017).Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth.Proceedings of the National Academy of Sciences of the United States of America,114(5).
MLA	Weller B.,et al."Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth".Proceedings of the National Academy of Sciences of the United States of America 114.5(2017).