气候变化领域集成服务门户(CCPortal): Myosin-driven transport network in plants

CCPortal

DOI	10.1073/pnas.1620577114
	Myosin-driven transport network in plants
	Kurth E.G.; Peremyslov V.V.; Turner H.L.; Makarova K.S.; Iranzo J.; Mekhedov S.L.; Koonin E.V.; Dolja V.V.
发表日期	2017
ISSN	0027-8424
起始页码	E1385
结束页码	E1394
卷号	114 期号:8
英文摘要	We investigate the myosin XI-driven transport network in Arabidopsis using protein-protein interaction, subcellular localization, gene knockout, and bioinformatics analyses. The two major groups of nodes in this network are myosins XI and their membrane-anchored receptors (MyoB) that, together, drive endomembrane trafficking and cytoplasmic streaming in the plant cells. The network shows high node connectivity and is dominated by generalists, with a smaller fraction of more specialized myosins and receptors. We show that interaction with myosins and association with motile vesicles are common properties of the MyoB family receptors. We identify previously uncharacterized myosin-binding proteins, putative myosin adaptors that belong to two unrelated families, with four members each (MadA and MadB). Surprisingly, MadA1 localizes to the nucleus and is rapidly transported to the cytoplasm, suggesting the existence of myosin XI-driven nucleocytoplasmic trafficking. In contrast, MadA2 and MadA3, as well as MadB1, partition between the cytosolic pools of motile endomembrane vesicles that colocalize with myosin XI-K and diffuse material that does not. Gene knockout analysis shows that MadB1-4 contribute to polarized root hair growth, phenocopying myosins, whereas MadA1-4 are redundant for this process. Phylogenetic analysis reveals congruent evolutionary histories of the myosin XI, MyoB, MadA, and MadB families. All these gene families emerged in green algae and show concurrent expansions via serial duplication in flowering plants. Thus, the myosin XI transport network increased in complexity and robustness concomitantly with the land colonization by flowering plants and, by inference, could have been a major contributor to this process. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Adaptors; Cytoplasmic streaming; Myosins; Nuclear transport; Receptors
语种	英语
scopus关键词	Arabidopsis protein; F actin; maltose binding protein; myosin; triton x 100; cell surface receptor; myosin; angiosperm; Arabidopsis; Article; cell fractionation; cellular distribution; coevolution; controlled study; cytoplasm; cytosol; DNA library; family; fluorescence; gene interaction; green alga; hair growth; microsome; molecular cloning; monocot; nonhuman; nucleolus; phenotype; phylogeny; plant genome; plant growth; priority journal; promoter region; protein localization; protein transport; quantitative analysis; root hair; stable expression; tetratricopeptide repeat; cytoplasmic streaming; metabolism; physiology; plant root; protein transport; Arabidopsis; Arabidopsis Proteins; Cytoplasmic Streaming; Myosins; Phylogeny; Plant Roots; Protein Transport; Receptors, Cell Surface
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160662
作者单位	Kurth, E.G., Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, United States; Peremyslov, V.V., Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, United States; Turner, H.L., Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, United States; Makarova, K.S., National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, United States; Iranzo, J., National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, United States; Mekhedov, S.L., National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, United States; Koonin, E.V., National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, United States; Dolja, V.V., Department of Bo...
推荐引用方式 GB/T 7714	Kurth E.G.,Peremyslov V.V.,Turner H.L.,et al. Myosin-driven transport network in plants[J],2017,114(8).
APA	Kurth E.G..,Peremyslov V.V..,Turner H.L..,Makarova K.S..,Iranzo J..,...&Dolja V.V..(2017).Myosin-driven transport network in plants.Proceedings of the National Academy of Sciences of the United States of America,114(8).
MLA	Kurth E.G.,et al."Myosin-driven transport network in plants".Proceedings of the National Academy of Sciences of the United States of America 114.8(2017).