Climate Change Data Portal
DOI | 10.1073/pnas.1618227114 |
Cooperation of two distinct coupling proteins creates chemosensory network connections | |
Abedrabbo S.; Castellon J.; Collins K.D.; Johnson K.S.; Ottemann K.M. | |
发表日期 | 2017 |
ISSN | 0027-8424 |
起始页码 | 2970 |
结束页码 | 2975 |
卷号 | 114期号:11 |
英文摘要 | Although it is appreciated that bacterial chemotaxis systems rely on coupling, also called scaffold, proteins to both connect input receptors with output kinases and build interkinase connections that allow signal amplification, it is not yet clear why many systems use more than one coupling protein. We examined the distinct functions for multiple coupling proteins in the bacterial chemotaxis system of Helicobacter pylori, which requires two nonredundant coupling proteins for chemotaxis: CheW and CheV1, a hybrid of a CheW and a phosphorylatable receiver domain. We report that CheV1 and CheW have largely redundant abilities to interact with chemoreceptors and the CheA kinase, and both similarly activated CheA's kinase activity. We discovered, however, that they are not redundant for formation of the higher order chemoreceptor arrays that are known to form via CheA - CheW interactions. In support of this possibility, we found that CheW and CheV1 interact with each other and with CheA independent of the chemoreceptors. Therefore, it seems that some microbes have modified array formation to require CheW and CheV1. Our data suggest that multiple coupling proteins may be used to provide flexibility in the chemoreceptor array formation. |
英文关键词 | Chemoreceptor arrays; Chemotaxis; Scaffold; Signal transduction |
语种 | 英语 |
scopus关键词 | CheA kinase; protein; bacterial protein; carrier protein; methyl accepting chemotaxis protein; protein binding; autophosphorylation; carboxy terminal sequence; chemoreceptor; chemosensitivity; chemotaxis; complex formation; Conference Paper; Helicobacter pylori; immunofluorescence; phosphorylation; polyacrylamide gel electrophoresis; priority journal; protein localization; protein protein interaction; signal transduction; Western blotting; amino acid sequence; chemistry; metabolism; physiology; signal transduction; Amino Acid Sequence; Bacterial Proteins; Carrier Proteins; Chemotaxis; Methyl-Accepting Chemotaxis Proteins; Phosphorylation; Protein Binding; Signal Transduction |
来源期刊 | Proceedings of the National Academy of Sciences of the United States of America |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/160654 |
作者单位 | Abedrabbo, S., Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064, United States; Castellon, J., Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064, United States; Collins, K.D., Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064, United States; Johnson, K.S., Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064, United States; Ottemann, K.M., Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064, United States |
推荐引用方式 GB/T 7714 | Abedrabbo S.,Castellon J.,Collins K.D.,et al. Cooperation of two distinct coupling proteins creates chemosensory network connections[J],2017,114(11). |
APA | Abedrabbo S.,Castellon J.,Collins K.D.,Johnson K.S.,&Ottemann K.M..(2017).Cooperation of two distinct coupling proteins creates chemosensory network connections.Proceedings of the National Academy of Sciences of the United States of America,114(11). |
MLA | Abedrabbo S.,et al."Cooperation of two distinct coupling proteins creates chemosensory network connections".Proceedings of the National Academy of Sciences of the United States of America 114.11(2017). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。