气候变化领域集成服务门户(CCPortal): Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa

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DOI	10.1073/pnas.1615575114
	Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa
	Racki L.R.; Tocheva E.I.; Dieterle M.G.; Sullivan M.C.; Jensen G.J.; Newman D.K.
发表日期	2017
ISSN	0027-8424
起始页码	E2440
结束页码	E2449
卷号	114 期号:12
英文摘要	Polyphosphate (polyP) granule biogenesis is an ancient and ubiquitous starvation response in bacteria. Although the ability to make polyP is important for survival during quiescence and resistance to diverse environmental stresses, granule genesis is poorly understood. Using quantitative microscopy at high spatial and temporal resolution, we show that granule genesis in Pseudomonas aeruginosa is tightly organized under nitrogen starvation. Following nucleation as many microgranules throughout the nucleoid, polyP granules consolidate and become transiently spatially organized during cell cycle exit. Between 1 and 3 h after nitrogen starvation, a minority of cells have divided, yet the total granule number per cell decreases, total granule volume per cell dramatically increases, and individual granules grow to occupy diameters as large as ∼200 nm. At their peak, mature granules constitute ∼2% of the total cell volume and are evenly spaced along the long cell axis. Following cell cycle exit, granules initially retain a tight spatial organization, yet their size distribution and spacing relax deeper into starvation. Mutant cells lacking polyP elongate during starvation and contain more than one origin. PolyP promotes cell cycle exit by functioning at a step after DNA replication initiation. Together with the universal starvation alarmone (p)ppGpp, polyP has an additive effect on nucleoid dynamics and organization during starvation. Notably, cell cycle exit is temporally coupled to a net increase in polyP granule biomass, suggesting that net synthesis, rather than consumption of the polymer, is important for the mechanism by which polyP promotes completion of cell cycle exit during starvation.
英文关键词	Biomineralization; Cell cycle; Nucleoid; Polyphosphate; Starvation
语种	英语
scopus关键词	bacterial DNA; nitrogen; polyphosphate; bacterial protein; polyphosphate; Article; bacterial cell; bacterial chromosome; biogenesis; cell cycle; cell division; cell mutant; cell nucleus; cell size; cell stress; cell volume; controlled study; DNA replication; microscopy; nonhuman; priority journal; Pseudomonas aeruginosa; quantitative analysis; cell cycle; chemistry; cytology; genetics; metabolism; Pseudomonas aeruginosa; Bacterial Proteins; Cell Cycle; Cell Division; Polyphosphates; Pseudomonas aeruginosa
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160652
作者单位	Racki, L.R., Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States; Tocheva, E.I., Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States; Dieterle, M.G., Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States; Sullivan, M.C., Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States; Jensen, G.J., Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, United States; Newman, D.K., Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, United States, Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, Uni...
推荐引用方式 GB/T 7714	Racki L.R.,Tocheva E.I.,Dieterle M.G.,et al. Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa[J],2017,114(12).
APA	Racki L.R.,Tocheva E.I.,Dieterle M.G.,Sullivan M.C.,Jensen G.J.,&Newman D.K..(2017).Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa.Proceedings of the National Academy of Sciences of the United States of America,114(12).
MLA	Racki L.R.,et al."Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa".Proceedings of the National Academy of Sciences of the United States of America 114.12(2017).