气候变化领域集成服务门户(CCPortal): Malate-dependent Fe accumulation is a critical checkpoint in the root developmental response to low phosphate

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DOI	10.1073/pnas.1701952114
	Malate-dependent Fe accumulation is a critical checkpoint in the root developmental response to low phosphate
	Mora-Macías J.; Ojeda-Rivera J.O.; Gutiérrez-Alanís D.; Yong-Villalobos L.; Oropeza-Aburto A.; Raya-González J.; Jiménez-Domínguez G.; Chávez-Calvillo G.; Rellán-Álvarez R.; Herrera-Estrell L.
发表日期	2017
ISSN	0027-8424
起始页码	E3563
结束页码	E3572
卷号	114 期号:17
英文摘要	Low phosphate (Pi) availability constrains plant development and seed production in both natural and agricultural ecosystems. When Pi is scarce, modifications of root system architecture (RSA) enhance the soil exploration ability of the plant and lead to an increase in Pi uptake. In Arabidopsis, an iron-dependent mechanism reprograms primary root growth in response to low Pi availability. This program is activated upon contact of the root tip with low-Pi media and induces premature cell differentiation and the arrest of mitotic activity in the root apical meristem, resulting in a short-root phenotype. However, the mechanisms that regulate the primary root response to Pilimiting conditions remain largely unknown. Here we report on the isolation and characterization of two low-Pi insensitive mutants (lpi5 and lpi6), which have a long-root phenotype when grown in low-Pi media. Cellular, genomic, and transcriptomic analysis of low-Pi insensitive mutants revealed that the genes previously shown to underlie Arabidopsis Al tolerance via root malate exudation, known as SENSITIVE TO PROTON RHIZOTOXICITY (STOP1) and ALUMINUM ACTIVATED MALATE TRANSPORTER 1 (ALMT1), represent a critical checkpoint in the root developmental response to Pi starvation in Arabidopsis thaliana. Our results also show that exogenous malate can rescue the long-root phenotype of lpi5 and lpi6. Malate exudation is required for the accumulation of Fe in the apoplast of meristematic cells, triggering the differentiation of meristematic cells in response to Pi deprivation.
英文关键词	Cell differentiation; Gene regulation; Iron; Phosphate; Root response
语种	英语
scopus关键词	aluminum activated malate transporter 1; iron; malic acid; membrane protein; phosphate; plant DNA; transcription factor; transcription factor stop1; unclassified drug; zinc finger protein; ALMT1 protein, Arabidopsis; Arabidopsis protein; iron; malic acid; malic acid derivative; organic anion transporter; phosphate; STOP1 protein, Arabidopsis; transcription factor; abiotic stress; allele; amino acid substitution; apoplast; Arabidopsis thaliana; Article; cell differentiation; environmental stress; gene control; gene mapping; gene mutation; genetic code; lpi5 gene; lpi6 gene; mutant; nonhuman; phenotype; plant cell; plant development; plant genome; priority journal; root growth; root system; transcriptomics; Arabidopsis; growth, development and aging; meristem; metabolism; Arabidopsis; Arabidopsis Proteins; Iron; Malates; Meristem; Organic Anion Transporters; Phosphates; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160641
作者单位	Mora-Macías, J., Laboratorio Nacional de Genómica para la Biodiversidad/Unidad de Genómica Avanzada, Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Guanajuato, 36821, Mexico; Ojeda-Rivera, J.O., Laboratorio Nacional de Genómica para la Biodiversidad/Unidad de Genómica Avanzada, Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Guanajuato, 36821, Mexico; Gutiérrez-Alanís, D., Laboratorio Nacional de Genómica para la Biodiversidad/Unidad de Genómica Avanzada, Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Guanajuato, 36821, Mexico; Yong-Villalobos, L., Laboratorio Nacional de Genómica para la Biodiversidad/Unidad de Genómica Avanzada, Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Guanajuato, 36821, Mexico; Oropeza-Aburto, A., Laboratorio Nacional de Genómica para la Biodiversidad/Unidad de Genómica Avanzada, Centro de Investigación y Estudios Avanz...
推荐引用方式 GB/T 7714	Mora-Macías J.,Ojeda-Rivera J.O.,Gutiérrez-Alanís D.,et al. Malate-dependent Fe accumulation is a critical checkpoint in the root developmental response to low phosphate[J],2017,114(17).
APA	Mora-Macías J..,Ojeda-Rivera J.O..,Gutiérrez-Alanís D..,Yong-Villalobos L..,Oropeza-Aburto A..,...&Herrera-Estrell L..(2017).Malate-dependent Fe accumulation is a critical checkpoint in the root developmental response to low phosphate.Proceedings of the National Academy of Sciences of the United States of America,114(17).
MLA	Mora-Macías J.,et al."Malate-dependent Fe accumulation is a critical checkpoint in the root developmental response to low phosphate".Proceedings of the National Academy of Sciences of the United States of America 114.17(2017).